BMRB Entry 4711

Title:
NMR backbone assignments of the cold-regulated RNA-binding protein, RbpA1, in the cyanobacterium, Anabaena variabilis M3
Deposition date:
2000-04-10
Original release date:
2000-09-25
Authors:
Morita, Hayato; Murakami, Toshihide; Uegaki, Koichi; Yamazaki, Toshio; Sato, Naoki; Kyogoku, Yoshimasa; Hayashi, Hidenori
Citation:

Citation: Morita, Eugene; Murakami, Toshihide; Uegaki, Koichi; Yamazaki, Toshio; Sato, Naoki; Kyogoku, Yoshimasa; Hayashi, Hidenori. "Letter to the Editor: NMR backbone assignments of the cold-regulated RNA-binding protein, RbpA1, in the cyanobacterium, Anabaena variabilis M3"  J. Biomol. NMR 17, 351-352 (2000).

Assembly members:

Assembly members:
RNA-binding protein, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Anabaena variabilis M3   Taxonomy ID: 1172   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena variabilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: peT-21b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts96
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA-binding protein1

Entities:

Entity 1, RNA-binding protein 101 residues - Formula weight is not available

1   SERILETYRVALGLYASNLEUSERTYRGLU
2   VALTHRGLNASPTHRLEUSERALAVALPHE
3   ALAGLUTYRGLYTHRVALLYSARGVALGLN
4   LEUPROTHRASPARGGLUTHRGLYGLNPRO
5   ARGGLYPHEGLYPHEVALGLUMETGLYSER
6   GLUALAGLUGLUALAALAALAILEGLUALA
7   LEUASPGLYALAGLUTRPMETGLYARGASP
8   LEULYSVALASNLYSALALYSPROARGGLU
9   ASPARGGLYPROSERGLYGLYASNARGGLY
10   GLYTYRGLYGLYGLYGLYGLYARGASNARG
11   TYR

Samples:

sample_1: RNA-binding protein, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%

cond_1: pH: 6.9; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N_HSQCnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available

Software:

NMRPipe v1.6, NIH - peak picking and assignments

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

DBJ BAA04563 BAB72698
GB ABA24239
REF WP_010994915
SP Q44560
AlphaFold Q44560

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks