BMRB Entry 4698
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4698
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Citation: Marlow, Michael; Chim, Nicholas; Brown, Christopher; Barnett, Joey; Krezel, Andrzej. "Letter to the Editor:
1H, 13C, and 15N backbone assignments of the ligand binding domain of
TGFb type II receptor" J. Biomol. NMR 17, 349-350 (2000).
Assembly members:
Transforming Growth Factor Beta type II receptor, polymer, 122 residues, 13823 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-32
Entity Sequences (FASTA):
Transforming Growth Factor Beta type II receptor: MHDRSKENGLQLPRLCKFCD
VKATTCSNQDQCTSNCNITS
ICEKNNEVCAAVWRRNDENV
TLETICHDPQKRLYGHMLDD
SSSEQCVMKEKKDDGGLMFM
CSCTGEECNDVLIFSAIDPH
KP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 224 |
| 1H chemical shifts | 221 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TbR-II | 1 |
Entities:
Entity 1, TbR-II 122 residues - 13823 Da.
| 1 | MET | HIS | ASP | ARG | SER | LYS | GLU | ASN | GLY | LEU | ||||
| 2 | GLN | LEU | PRO | ARG | LEU | CYS | LYS | PHE | CYS | ASP | ||||
| 3 | VAL | LYS | ALA | THR | THR | CYS | SER | ASN | GLN | ASP | ||||
| 4 | GLN | CYS | THR | SER | ASN | CYS | ASN | ILE | THR | SER | ||||
| 5 | ILE | CYS | GLU | LYS | ASN | ASN | GLU | VAL | CYS | ALA | ||||
| 6 | ALA | VAL | TRP | ARG | ARG | ASN | ASP | GLU | ASN | VAL | ||||
| 7 | THR | LEU | GLU | THR | ILE | CYS | HIS | ASP | PRO | GLN | ||||
| 8 | LYS | ARG | LEU | TYR | GLY | HIS | MET | LEU | ASP | ASP | ||||
| 9 | SER | SER | SER | GLU | GLN | CYS | VAL | MET | LYS | GLU | ||||
| 10 | LYS | LYS | ASP | ASP | GLY | GLY | LEU | MET | PHE | MET | ||||
| 11 | CYS | SER | CYS | THR | GLY | GLU | GLU | CYS | ASN | ASP | ||||
| 12 | VAL | LEU | ILE | PHE | SER | ALA | ILE | ASP | PRO | HIS | ||||
| 13 | LYS | PRO |
Samples:
sample_1: Transforming Growth Factor Beta type II receptor, [U-13C; U-15N], 0.5 – 2 mM
sample_2: Transforming Growth Factor Beta type II receptor, [U-15N], 0.5 – 2 mM
all_samples: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | not available | not available | all_samples |
| HNCO | not available | not available | all_samples |
| HN(CO)CA | not available | not available | all_samples |
| CBCANH | not available | not available | all_samples |
| CBCA(CO)NH | not available | not available | all_samples |
| 15N edited TOCSY | not available | not available | all_samples |
Software:
Felix v1997 - processing
XEASY v1.3.13 - analysis, bookkeeping
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DMX 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks