BMRB Entry 4698

Title:
1H, 13C, and 15N backbone assignments of TGF-beta type II receptor ligand binding domain
Deposition date:
2000-03-22
Original release date:
2000-09-25
Authors:
Marlow, Michael; Chim, Nicholas; Brown, Christopher; Barnett, Joey; Krezel, Andrzej
Citation:

Citation: Marlow, Michael; Chim, Nicholas; Brown, Christopher; Barnett, Joey; Krezel, Andrzej. "Letter to the Editor: 1H, 13C, and 15N backbone assignments of the ligand binding domain of TGFb type II receptor"  J. Biomol. NMR 17, 349-350 (2000).

Assembly members:

Assembly members:
Transforming Growth Factor Beta type II receptor, polymer, 122 residues, 13823 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts224
1H chemical shifts221
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TbR-II1

Entities:

Entity 1, TbR-II 122 residues - 13823 Da.

1   METHISASPARGSERLYSGLUASNGLYLEU
2   GLNLEUPROARGLEUCYSLYSPHECYSASP
3   VALLYSALATHRTHRCYSSERASNGLNASP
4   GLNCYSTHRSERASNCYSASNILETHRSER
5   ILECYSGLULYSASNASNGLUVALCYSALA
6   ALAVALTRPARGARGASNASPGLUASNVAL
7   THRLEUGLUTHRILECYSHISASPPROGLN
8   LYSARGLEUTYRGLYHISMETLEUASPASP
9   SERSERSERGLUGLNCYSVALMETLYSGLU
10   LYSLYSASPASPGLYGLYLEUMETPHEMET
11   CYSSERCYSTHRGLYGLUGLUCYSASNASP
12   VALLEUILEPHESERALAILEASPPROHIS
13   LYSPRO

Samples:

sample_1: Transforming Growth Factor Beta type II receptor, [U-13C; U-15N], 0.5 – 2 mM

sample_2: Transforming Growth Factor Beta type II receptor, [U-15N], 0.5 – 2 mM

all_samples: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availableall_samples
HNCOnot availablenot availableall_samples
HN(CO)CAnot availablenot availableall_samples
CBCANHnot availablenot availableall_samples
CBCA(CO)NHnot availablenot availableall_samples
15N edited TOCSYnot availablenot availableall_samples

Software:

Felix v1997 - processing

XEASY v1.3.13 - analysis, bookkeeping

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
GB AAA49091
REF NP_990759
SP Q90999
AlphaFold Q90999

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks