BMRB Entry 4689

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4689

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Title:
NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: a Comparison of High and Low pH Conformations
Deposition date:
2000-03-16
Original release date:
2002-04-01
Authors:
Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens
Citation:

Citation: Kasimova, Marina; Kristensen, Soren; Howe, Peter; Christensen, Thorkild; Matthiesen, Finn; Petersen, Jorgen; Sorensen, Hans; Led, Jens. "NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: A Comparison of high and low pH Conformations"  J. Mol. Biol. 318, 679-695 (2002).
PubMed: 12054815

Assembly members:

Assembly members:
Human Growth Hormone, polymer, 191 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human Growth Hormone: FPTIPLSRLFDNAMLRAHRL HQLAFDTYQEFEEAYIPKEQ KYSFLQNPQTSLCFSESIPT PSNREETQQKSNLELLRISL LLIQSWLEPVQFLRSVFANS LVYGASDSNVYDLLKDLEEG IQTLMGRLEDGSPRTGQIFK QTYSKFDTNSHNDDALLKNY GLLYCFRKDMDKVETFLRIV QCRSVEGSCGF

Data sets:
Data typeCount
1H chemical shifts502
13C chemical shifts402
15N chemical shifts245
heteronuclear NOE values237
T1 relaxation values237
T2 relaxation values237
order parameters237

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hGH1

Entities:

Entity 1, hGH 191 residues - Formula weight is not available

1   PHEPROTHRILEPROLEUSERARGLEUPHE
2   ASPASNALAMETLEUARGALAHISARGLEU
3   HISGLNLEUALAPHEASPTHRTYRGLNGLU
4   PHEGLUGLUALATYRILEPROLYSGLUGLN
5   LYSTYRSERPHELEUGLNASNPROGLNTHR
6   SERLEUCYSPHESERGLUSERILEPROTHR
7   PROSERASNARGGLUGLUTHRGLNGLNLYS
8   SERASNLEUGLULEULEUARGILESERLEU
9   LEULEUILEGLNSERTRPLEUGLUPROVAL
10   GLNPHELEUARGSERVALPHEALAASNSER
11   LEUVALTYRGLYALASERASPSERASNVAL
12   TYRASPLEULEULYSASPLEUGLUGLUGLY
13   ILEGLNTHRLEUMETGLYARGLEUGLUASP
14   GLYSERPROARGTHRGLYGLNILEPHELYS
15   GLNTHRTYRSERLYSPHEASPTHRASNSER
16   HISASNASPASPALALEULEULYSASNTYR
17   GLYLEULEUTYRCYSPHEARGLYSASPMET
18   ASPLYSVALGLUTHRPHELEUARGILEVAL
19   GLNCYSARGSERVALGLUGLYSERCYSGLY
20   PHE

Samples:

sample_1: Human Growth Hormone, [U-90% 15N; U-95% 13C], 0.9 mM; H2O 10%; D2O 90%

sample_2: Human Growth Hormone, [U-90% 15N], 0.9 mM; H2O 10%; D2O 90%

sample_3: Human Growth Hormone, [Leu-90% 15N], 0.9 mM; H2O 10%; D2O 90%; Sodium phosphate 10 mM

sample_4: Human Growth Hormone, [U-90% 15N; U-95% 13C], 0.9 mM; H2O 0.04%; D2O 99.96%

sample_conditions_one: pH: 2.7; temperature: 305 K; pressure: 1 atm

sample_conditions_two: pH: 7.0; temperature: 305 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AM500 500 MHz
  • Varian UnityINOVA 500 MHz
  • Varian UnityINOVA 750 MHz
  • Varian UnityINOVA 800 MHz

Related Database Links:

BMRB 25029
PDB
DBJ BAJ21175
EMBL CAA23778 CAA23779
GB AAA52549 AAA72260 AAA98618 AAC42099 AAF23135
PRF 1403262B
REF NP_000506 NP_001184093 NP_001277233 XP_002827754 XP_003262698
SP P01241 P58756
TPE CDW51387 CDW51389 CDW51391 CDW51396 CDW51399
AlphaFold P01241 P58756

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks