BMRB Entry 4688
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4688
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Woestenenk, Esmeralda; Allard, Peter; Gongadze, George; Moskalenko, Svetlana; Shcherbakov, Dmitry; Rak, Alexey; Garber, Maria; Hard, Torleif; Berglund, Helena. "Letter to the Editor:
Assignment and secondary structure identification of the ribosomal
protein L18 from Thermus thermophilus" J. Biomol. NMR 17, 273-274 (2000).
Assembly members:
L18, polymer, 111 residues, 12480 Da.
Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11C
Entity Sequences (FASTA):
L18: ARLTAYERRKFRVRNRIKRT
GRLRLSVFRSLKHIYAQIID
DEKGVTLVSASSLALKLKGN
KTEVARQVGRALAEKALALG
IKQVAFDRGPYKYHGRVKAL
AEGAREGGLEF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 704 |
| 13C chemical shifts | 343 |
| 15N chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L18 | 1 |
Entities:
Entity 1, L18 111 residues - 12480 Da.
| 1 | ALA | ARG | LEU | THR | ALA | TYR | GLU | ARG | ARG | LYS | ||||
| 2 | PHE | ARG | VAL | ARG | ASN | ARG | ILE | LYS | ARG | THR | ||||
| 3 | GLY | ARG | LEU | ARG | LEU | SER | VAL | PHE | ARG | SER | ||||
| 4 | LEU | LYS | HIS | ILE | TYR | ALA | GLN | ILE | ILE | ASP | ||||
| 5 | ASP | GLU | LYS | GLY | VAL | THR | LEU | VAL | SER | ALA | ||||
| 6 | SER | SER | LEU | ALA | LEU | LYS | LEU | LYS | GLY | ASN | ||||
| 7 | LYS | THR | GLU | VAL | ALA | ARG | GLN | VAL | GLY | ARG | ||||
| 8 | ALA | LEU | ALA | GLU | LYS | ALA | LEU | ALA | LEU | GLY | ||||
| 9 | ILE | LYS | GLN | VAL | ALA | PHE | ASP | ARG | GLY | PRO | ||||
| 10 | TYR | LYS | TYR | HIS | GLY | ARG | VAL | LYS | ALA | LEU | ||||
| 11 | ALA | GLU | GLY | ALA | ARG | GLU | GLY | GLY | LEU | GLU | ||||
| 12 | PHE |
Samples:
sample_1: L18, [U-13C; U-15N], 0.7 – 1.6 mM
conditions_1: pH: 5.9; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | conditions_1 |
| HN(CO)CA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| CBCA(CO)NH | sample_1 | not available | conditions_1 |
| (H)C(CO)NH | sample_1 | not available | conditions_1 |
| H(CCO)NH | sample_1 | not available | conditions_1 |
| HCCH-TOCSY | sample_1 | not available | conditions_1 |
| HCCH-COSY | sample_1 | not available | conditions_1 |
| 1H-15N-TOCSY | sample_1 | not available | conditions_1 |
| 1H-15N-NOESY | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD71499 |
| EMBL | CAA62289 |
| GB | AAS81654 AEG34089 AFH38278 EIA38537 |
| REF | WP_008633391 YP_144942 |
| SP | P80320 Q5SHQ4 Q72I20 |
| AlphaFold | P80320 Q5SHQ4 Q72I20 |
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