BMRB Entry 4678

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR4678

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RNA Polymerase Subunit RPB5 from Methanobacterium thermoautotrophicum

Deposition date:

2000-03-03

Original release date:

2000-12-18

Authors:

Yee, Adelinda; Booth, Valerie; Dharamsi, Akil; Engel, Asaph; Edwards, Aled; Arrowsmith, Cheryl

Citation:

Citation: Yee, Adelinda; Booth, Valerie; Dharamsi, Akil; Engel, Asaph; Edwards, Aled; Arrowsmith, Cheryl. "Solution Structure of the RNA Polymerase Subunit RPB5 from Methanobacterium thermoautotrophicum" Proc. Natl. Acad. Sci. U. S. A. 97, 6311-6315 (2000).

Assembly members:

Assembly members:
rna polymerase subunit 5, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Methanobacterium thermoautotrophicum Taxonomy ID: 2166 Superkingdom: Archaea Kingdom: not available Genus/species: Methanobacterium thermoautotrophicum

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rna polymerase subunit 5: MKREILKHQLVPEHVILNES EAKRVLKELDAHPEQLPKIK TTDPVAKAIGAKRGDIVKII RKSPTAEEFVTYRLVQD

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
1H chemical shifts	566
13C chemical shifts	269
15N chemical shifts	70

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rpb5	1

Entities:

Entity 1, rpb5 77 residues - Formula weight is not available

1

MET

LYS

ARG

GLU

ILE

LEU

LYS

HIS

GLN

LEU

2

VAL

PRO

GLU

HIS

VAL

ILE

LEU

ASN

GLU

SER

3

GLU

ALA

LYS

ARG

VAL

LEU

LYS

GLU

LEU

ASP

4

ALA

HIS

PRO

GLU

GLN

LEU

PRO

LYS

ILE

LYS

5

THR

ASP

PRO

VAL

ALA

LYS

ALA

ILE

GLY

6

ALA

LYS

ARG

GLY

ASP

ILE

VAL

LYS

ILE

7

ARG

LYS

SER

PRO

THR

ALA

GLU

PHE

VAL

8

THR

TYR

ARG

LEU

VAL

GLN

ASP

Samples:

sample_one: rna polymerase subunit 5, [U-13C; U-15N], 1 mM

sample_conditions_one: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	sample_one	not available	sample_conditions_one
HBCBCACONNH	sample_one	not available	sample_conditions_one
HCCHTOCSY	sample_one	not available	sample_conditions_one
HCCTOCSY	sample_one	not available	sample_conditions_one
HNCO	sample_one	not available	sample_conditions_one
CCC-TOCSY	sample_one	not available	sample_conditions_one
15N-DIPSI	sample_one	not available	sample_conditions_one

Software:

nmrview v3.0 - peak picking

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz

PDB	1EIK
DBJ	BAM70200
GB	AAB85539
REF	WP_010876674 WP_048175656
SP	O27122
AlphaFold	O27122