BMRB Entry 4677

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PDB ID: 1exk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4677
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ

Deposition date:

2000-03-02

Original release date:

2001-05-03

Authors:

Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane

Citation:

Citation: Martinez-Yamout, Maria; Legge, Glen; Zhang, Ouwen; Wright, Peter; Dyson, Jane. "Solution Structure of the Cysteine-rich Domain of the Escherichia coli Chaperone Protein DnaJ" J. Mol. Biol. 300, 805-818 (2000).

Assembly members:

Assembly members:
cysteine-rich domain of DnaJ, polymer, 79 residues, 8518 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cysteine-rich domain of DnaJ: GVTKEIRIPTLEECDVCHGS GAKPGTQPQTCPTCHGSGQV QMRQGFFAVQQTCPHCQGRG TLIKDPCNKCHGHGRVERS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	300
15N chemical shifts	81
1H chemical shifts	482

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CRD monomer	1
2	Zn1	2
3	Zn2	2

Entities:

Entity 1, CRD monomer 79 residues - 8518 Da.

1

GLY

VAL

THR

LYS

GLU

ILE

ARG

ILE

PRO

THR

2

LEU

GLU

CYS

ASP

VAL

CYS

HIS

GLY

SER

3

GLY

ALA

LYS

PRO

GLY

THR

GLN

PRO

GLN

THR

4

CYS

PRO

THR

CYS

HIS

GLY

SER

GLY

GLN

VAL

5

GLN

MET

ARG

GLN

GLY

PHE

ALA

VAL

GLN

6

GLN

THR

CYS

PRO

HIS

CYS

GLN

GLY

ARG

GLY

7

THR

LEU

ILE

LYS

ASP

PRO

CYS

ASN

LYS

CYS

8

HIS

GLY

HIS

GLY

ARG

VAL

GLU

ARG

SER

Entity 2, Zn1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: cysteine-rich domain of DnaJ, [U-95% 13C; U-90% 15N], 0.6 mM

ex-cond_1: pH: 7.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCA(CO)NH	sample_1	not available	ex-cond_1
HNCACB	sample_1	not available	ex-cond_1
C(CO)NH-TOOCSY	sample_1	not available	ex-cond_1
HNCO	sample_1	not available	ex-cond_1
HCCH-TOCSY	sample_1	not available	ex-cond_1
15N-edited NOESY	sample_1	not available	ex-cond_1
15N-edited TOCSY	sample_1	not available	ex-cond_1
13C-edited NOESY	sample_1	not available	ex-cond_1
13C, 15N-edited NOESY	sample_1	not available	ex-cond_1

Software:

Felix v97 - transform and plotting

NMRPipe - transform

NMRView - plotting and analysis

NMR spectrometers:

Brruker DMX 750 MHz
Brruker DRX 600 MHz
Brruker AMX 500 MHz

PDB	1EXK
DBJ	BAB33438 BAB96590 BAF79748 BAF79749 BAF79793
GenBank	AAA00009 AAA23693 AAC73126 AAG54315 AAN41681
REF	AP_000679 NP_285707 NP_308042 NP_414556 NP_705974
SWISS-PROT	A7ZHA5 A7ZVV8 B1IRF9 P08622 Q0T8H5