BMRB Entry 4673
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4673
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Citation: Luecke, Christian; Zhang, Fengli; Hamilton, James; Sacchettini, James; Rueterjans, Heinz. "Solution structure of Ileal Lipid Binding Protein in complex with glycocholate" Eur. J. Biochem. 267, 2929-2938 (2000).
Assembly members:
ileal lipid binding protein, polymer, 127 residues, 14088 Da.
GCH, non-polymer, 465.623 Da.
Natural source: Common Name: pig Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ileal lipid binding protein: AFTGKYEIESEKNYDEFMKR
LALPSDAIDKARNLKIISEV
KQDGQNFTWSQQYPGGHSIT
NTFTIGKECDIETIGGKKFK
ATVQMEGGKVVVNSPNYHHT
AEIVDGKLVEVSTVGGVSYE
RVSKKLA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 901 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ILBP | 1 |
| 2 | glycocholate | 2 |
Entities:
Entity 1, ILBP 127 residues - 14088 Da.
| 1 | ALA | PHE | THR | GLY | LYS | TYR | GLU | ILE | GLU | SER | ||||
| 2 | GLU | LYS | ASN | TYR | ASP | GLU | PHE | MET | LYS | ARG | ||||
| 3 | LEU | ALA | LEU | PRO | SER | ASP | ALA | ILE | ASP | LYS | ||||
| 4 | ALA | ARG | ASN | LEU | LYS | ILE | ILE | SER | GLU | VAL | ||||
| 5 | LYS | GLN | ASP | GLY | GLN | ASN | PHE | THR | TRP | SER | ||||
| 6 | GLN | GLN | TYR | PRO | GLY | GLY | HIS | SER | ILE | THR | ||||
| 7 | ASN | THR | PHE | THR | ILE | GLY | LYS | GLU | CYS | ASP | ||||
| 8 | ILE | GLU | THR | ILE | GLY | GLY | LYS | LYS | PHE | LYS | ||||
| 9 | ALA | THR | VAL | GLN | MET | GLU | GLY | GLY | LYS | VAL | ||||
| 10 | VAL | VAL | ASN | SER | PRO | ASN | TYR | HIS | HIS | THR | ||||
| 11 | ALA | GLU | ILE | VAL | ASP | GLY | LYS | LEU | VAL | GLU | ||||
| 12 | VAL | SER | THR | VAL | GLY | GLY | VAL | SER | TYR | GLU | ||||
| 13 | ARG | VAL | SER | LYS | LYS | LEU | ALA |
Entity 2, glycocholate - C26 H43 N O6 - 465.623 Da.
| 1 | GCH |
Samples:
sample_1: ileal lipid binding protein3 – 4 mM
cond1: pH: 5.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | sample_1 | not available | cond1 |
| 1H-1H NOESY | sample_1 | not available | cond1 |
| 1H-13C NOESY | sample_1 | not available | cond1 |
Software:
XWINNMR v1.3 - data acquisition and processing
AURELIA v2.1 - data analysis
FELIX v95.0 - data analysis
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DRX 800 MHz