BMRB Entry 4666

Name: Biotin carboxyl carrier domain of transcarboxylase (TC 1.3S)
Published: 2000-05-26

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PDB ID: 1dcz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4666
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Biotin carboxyl carrier domain of transcarboxylase (TC 1.3S)

Deposition date:

2000-02-08

Original release date:

2000-05-26

Authors:

Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.

Citation:

Citation: Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.. "High Resolution Solution Structure of the 1.3S Subunit of Transcarboxylase from Propionibacterium shermanii" Biochemistry 39, 2509-2516 (2000).

Assembly members:

Assembly members:
TRANSCARBOXYLASE 1.3S SUBUNIT, polymer, 77 residues, 12367 Da.
NORBIOTIN, non-polymer, 230.284 Da.

Natural source:

Natural source: Common Name: Propionibacterium Freudenreichii subsp shermanii Taxonomy ID: 1752 Superkingdom: Bacteria Kingdom: not available Genus/species: Propionibacterium Freudenreichii subsp shermanii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TRANSCARBOXYLASE 1.3S SUBUNIT: AGAGKAGEGEIPAPLAGTVS KILVKEGDTVKAGQTVLVLE AMKMETEINAPTDGKVEKVL VKERDAVQGGQGLIKIG

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- coupling_constants

Data type	Count
15N chemical shifts	80
1H chemical shifts	545
13C chemical shifts	247
coupling constants	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	transcarboxylase 1.3S subunit	1
2	biotin moiety	2

Entities:

Entity 1, transcarboxylase 1.3S subunit 77 residues - 12367 Da.

1

ALA

GLY

ALA

GLY

LYS

ALA

GLY

GLU

GLY

GLU

2

ILE

PRO

ALA

PRO

LEU

ALA

GLY

THR

VAL

SER

3

LYS

ILE

LEU

VAL

LYS

GLU

GLY

ASP

THR

VAL

4

LYS

ALA

GLY

GLN

THR

VAL

LEU

VAL

LEU

GLU

5

ALA

MET

LYS

MET

GLU

THR

GLU

ILE

ASN

ALA

6

PRO

THR

ASP

GLY

LYS

VAL

GLU

LYS

VAL

LEU

7

VAL

LYS

GLU

ARG

ASP

ALA

VAL

GLN

GLY

8

GLN

GLY

LEU

ILE

LYS

ILE

GLY

Entity 2, biotin moiety - C9 H14 N2 O3 S - 230.284 Da.

1

SNR

Samples:

sample_1: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-95%13C; U-90%15N], 1 – 2 mM

sample_2: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-90% 15N], 1 – 2 mM

sample_cond_1: pH: 6.7; temperature: 293 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_cond_1

Software:

NMRPIPE vSGI6X.M4 - processing

PIPP v3.7.3 - data analysis

X-PLOR v3.81 - structure solution

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz

BMRB	4667
PDB	1DCZ 1DD2 1O78
EMBL	CAD59402 CBL57376 CDP49157 CEG86413 CEG88429
GB	AAA25674 AAA89090 AAA89092 AAA89094 AJQ91496
REF	WP_013161729 WP_048769525
SP	P02904
AlphaFold	P02904 P02904