BMRB Entry 4661

Name: Solution structure of APAF-1 CARD
Published: 2000-03-07

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PDB ID: 1c15
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4661
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of APAF-1 CARD

Deposition date:

2000-02-02

Original release date:

2000-03-07

Authors:

Zhou, Pei; Chou, James; Sanchez-Olea, Roberto; Yuan, Junying; Wagner, Gerhard

Citation:

Citation: Zhou, Pei; Chou, James; Sanchez-Olea, Roberto; Yuan, Junying; Wagner, Gerhard. "Solution structure of Apaf-1 CARD and its interaction with caspase-9 CARD: A structural basis for specific adaptor/caspase interaction" Proc. Natl. Acad. Sci. U.S.A. 96, 11265-11270 (1999).

Assembly members:

Assembly members:
APOPTOTIC PROTEASE ACTIVATING FACTOR 1, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APOPTOTIC PROTEASE ACTIVATING FACTOR 1: MDAKARNCLLQHREALEKDI KTSYIMDHMISDGFLTISEE EKVRNEPTQQQRAAMLIKMI LKKDNDSYVSFYNALLHEGY KDLAALLHDGIPVVSSS

Data sets:

assigned_chemical_shifts
- set_1

Data type	Count
15N chemical shifts	90
1H chemical shifts	627
13C chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Apaf-1 CARD	1

Entities:

Entity 1, Apaf-1 CARD 97 residues - Formula weight is not available

1

MET

ASP

ALA

LYS

ALA

ARG

ASN

CYS

LEU

2

GLN

HIS

ARG

GLU

ALA

LEU

GLU

LYS

ASP

ILE

3

LYS

THR

SER

TYR

ILE

MET

ASP

HIS

MET

ILE

4

SER

ASP

GLY

PHE

LEU

THR

ILE

SER

GLU

5

GLU

LYS

VAL

ARG

ASN

GLU

PRO

THR

GLN

6

GLN

ARG

ALA

MET

LEU

ILE

LYS

MET

ILE

7

LEU

LYS

ASP

ASN

ASP

SER

TYR

VAL

SER

8

PHE

TYR

ASN

ALA

LEU

HIS

GLU

GLY

TYR

9

LYS

ASP

LEU

ALA

LEU

HIS

ASP

GLY

10

ILE

PRO

VAL

SER

Samples:

sample_1: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-15N], 0.5 – 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_2: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-13C], 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_3: APOPTOTIC PROTEASE ACTIVATING FACTOR 1 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_4: APOPTOTIC PROTEASE ACTIVATING FACTOR 1, [U-13C; U-15N; U-80% 2H], 1 mM; phosphate buffer 20 mM; NaCl 50 mM; DTT 5 mM

sample_cond_1: pH: 6.5; temperature: 300 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	not available	not available	sample_cond_1
3D_13C-separated_NOESY	not available	not available	sample_cond_1
2D NOESY	not available	not available	sample_cond_1
HMQC-J	not available	not available	sample_cond_1

Software:

DYANA v1.5 - Structure solution

X-PLOR v3.851 - refinement

NMR spectrometers:

Bruker DMX 500 MHz

PDB	1C15 1CWW 1CY5 1Z6T 2P1H 2YGS 3J2T 3YGS 4RHW
DBJ	BAA24843 BAC77343 BAJ20211
EMBL	CAB55579 CAB55580 CAB55581 CAB55582 CAB55583
GB	AAC51678 AAD34016 AAD38344 AAI36532 AAI36533
REF	NP_001151 NP_037361 NP_863651 NP_863658 NP_863659
SP	O14727
AlphaFold	O14727