BMRB Entry 4644

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PDB ID: 1fv5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4644
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions

Deposition date:

2000-09-18

Original release date:

2001-05-09

Authors:

Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.

Citation:

Citation: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.. "Solution structures of two CCHC zinc fingers from the FOG family protein U-shaped that mediate protein-protein interactions" Structure Fold Des. 8, 1157-1166 (2000).
PubMed: 11080638

Assembly members:

Assembly members:
FIRST ZINC FINGER OF U-SHAPED, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FIRST ZINC FINGER OF U-SHAPED: GSLLKPARFMCLPCGIAFSS PSTLEAHQAYYCSHRI

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- JHNHA

Data type	Count
1H chemical shifts	215
15N chemical shifts	32
coupling constants	28

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FIRST ZINC FINGER OF U-SHAPED	1
2	ZINC ION	2

Entities:

Entity 1, FIRST ZINC FINGER OF U-SHAPED 36 residues - Formula weight is not available

1

GLY

SER

LEU

LYS

PRO

ALA

ARG

PHE

MET

2

CYS

LEU

PRO

CYS

GLY

ILE

ALA

PHE

SER

3

PRO

SER

THR

LEU

GLU

ALA

HIS

GLN

ALA

TYR

4

TYR

CYS

SER

HIS

ARG

ILE

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: FIRST ZINC FINGER OF U-SHAPED 0.7 mM; H2O 95%; D2O 5%

sample_2: FIRST ZINC FINGER OF U-SHAPED, [U-15N], 0.7 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 5.5; temperature: 298 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1
HNHB	not available	not available	sample_cond_1

Software:

XWINNMR v2.6 - collection

XEASY v1.2 - data analysis

DYANA v1.5 - structure solution

CNS v1.0 - refinement

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1FV5 1Y0J 2L6Z
EMBL	CAA72991
GB	AAF51488 AAT94507 ACO56241 AGB92365 AGB92366
REF	NP_001259828 NP_001259829 NP_001259830 NP_001259831 NP_476780
SP	Q9VPQ6
AlphaFold	Q9VPQ6