BMRB Entry 4634
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4634
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Jeong, E.-J.; Hwang, G.-S.; Kim, K.; Kim, M.; Kim, S.; Kim, K.-S.. "Structural analysis of multi-functional peptide motifs present in human
bifunctional tRNA synthetase: Identification of RNA-binding residues and
functional implications for tandem repeats" Biochemistry 39, 15775-15782 (2000).
PubMed: 11123902
Assembly members:
Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17), polymer, 57 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17): DSLVLYNRVAVQGDVVRELK
AKKAPKEDVDAAVKQLLSLK
AEYKEKTGQEYKPGNPP
- coupling_constants
| Data type | Count |
| coupling constants | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Multifunctional aminoacyl-tRNA synthetase | 1 |
Entities:
Entity 1, Multifunctional aminoacyl-tRNA synthetase 57 residues - Formula weight is not available
| 1 | ASP | SER | LEU | VAL | LEU | TYR | ASN | ARG | VAL | ALA | ||||
| 2 | VAL | GLN | GLY | ASP | VAL | VAL | ARG | GLU | LEU | LYS | ||||
| 3 | ALA | LYS | LYS | ALA | PRO | LYS | GLU | ASP | VAL | ASP | ||||
| 4 | ALA | ALA | VAL | LYS | GLN | LEU | LEU | SER | LEU | LYS | ||||
| 5 | ALA | GLU | TYR | LYS | GLU | LYS | THR | GLY | GLN | GLU | ||||
| 6 | TYR | LYS | PRO | GLY | ASN | PRO | PRO |
Samples:
sample_1: Multifunctional aminoacyl-tRNA synthetase(E.C.6.1.1.17), [U-15N], 2 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 5.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| HNHA | sample_1 | not available | sample_cond_1 |
Software:
NMRPipe v1.7 - processing
PIPP v4.2.5 - data analysis
X-PLOR v3.851 - structure solution
NMR spectrometers:
- Varian UNITY 600 MHz
Related Database Links:
| PDB |