BMRB Entry 4632
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4632
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NMR-STAR v3 text file.
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Citation: Suzuki, M.; Hsu, Y.-T.; Youle, R.; Tjandra, N.. "Structure of Bax: Co-Regulation of Dimer Formation and Intracellular
Localization " Cell 103, 645-654 (2000).
PubMed: 11106734
Assembly members:
PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA), polymer, 192 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA): MDGSGEQPRGGGPTSSEQIM
KTGALLLQGFIQDRAGRMGG
EAPELALDPVPQDASTKKLS
ECLKRIGDELDSNMELQRMI
AAVDTDSPREVFFRVAADMF
SDGNFNWGRVVALFYFASKL
VLKALCTKVPELIRTIMGWT
LDFLRERLLGWIQDQGGWDG
LLSYFGTPTWQTVTIFVAGV
LTASLTIWKKMG
| Data type | Count |
| residual dipolar couplings | 604 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BAX | 1 |
Entities:
Entity 1, BAX 192 residues - Formula weight is not available
| 1 | MET | ASP | GLY | SER | GLY | GLU | GLN | PRO | ARG | GLY | ||||
| 2 | GLY | GLY | PRO | THR | SER | SER | GLU | GLN | ILE | MET | ||||
| 3 | LYS | THR | GLY | ALA | LEU | LEU | LEU | GLN | GLY | PHE | ||||
| 4 | ILE | GLN | ASP | ARG | ALA | GLY | ARG | MET | GLY | GLY | ||||
| 5 | GLU | ALA | PRO | GLU | LEU | ALA | LEU | ASP | PRO | VAL | ||||
| 6 | PRO | GLN | ASP | ALA | SER | THR | LYS | LYS | LEU | SER | ||||
| 7 | GLU | CYS | LEU | LYS | ARG | ILE | GLY | ASP | GLU | LEU | ||||
| 8 | ASP | SER | ASN | MET | GLU | LEU | GLN | ARG | MET | ILE | ||||
| 9 | ALA | ALA | VAL | ASP | THR | ASP | SER | PRO | ARG | GLU | ||||
| 10 | VAL | PHE | PHE | ARG | VAL | ALA | ALA | ASP | MET | PHE | ||||
| 11 | SER | ASP | GLY | ASN | PHE | ASN | TRP | GLY | ARG | VAL | ||||
| 12 | VAL | ALA | LEU | PHE | TYR | PHE | ALA | SER | LYS | LEU | ||||
| 13 | VAL | LEU | LYS | ALA | LEU | CYS | THR | LYS | VAL | PRO | ||||
| 14 | GLU | LEU | ILE | ARG | THR | ILE | MET | GLY | TRP | THR | ||||
| 15 | LEU | ASP | PHE | LEU | ARG | GLU | ARG | LEU | LEU | GLY | ||||
| 16 | TRP | ILE | GLN | ASP | GLN | GLY | GLY | TRP | ASP | GLY | ||||
| 17 | LEU | LEU | SER | TYR | PHE | GLY | THR | PRO | THR | TRP | ||||
| 18 | GLN | THR | VAL | THR | ILE | PHE | VAL | ALA | GLY | VAL | ||||
| 19 | LEU | THR | ALA | SER | LEU | THR | ILE | TRP | LYS | LYS | ||||
| 20 | MET | GLY |
Samples:
sample_1: PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA), [U-15N; U-13C], 1 mM; tris-acetate 10 mM; DTT 2 mM
sample_cond_1: pH: 6.00; temperature: 305.00 K; ionic strength: 0.01 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D 13C/15N-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 3D 15N-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
Software:
X-PLOR v3.840 - refinement
NMRPIPE v1.7 REV (1999.039.11.31) - structure solution
PIPP v4.1.5 - structure solution
UXNMR v2.5 - structure solution
NMR spectrometers:
- BRUKER DRX 800 MHz
Related Database Links:
| BMRB | 18340 |
| PDB | |
| DBJ | BAC53619 BAF83765 BAI46883 |
| EMBL | CAD10744 CCF78739 |
| GB | AAA03619 AAA03620 AAC48806 AAD22706 AAF82094 |
| REF | NP_001003011 NP_001247945 NP_001278357 NP_001278358 NP_001278360 |
| SP | O02703 Q07812 |
| TPG | DAA19512 |
| AlphaFold | O02703 Q07812 |