BMRB Entry 4593
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4593
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kingston, Richard; Fitzon-Ostendorp, Tanja; Eisenmesser, Elan; Schatz, Gisela; Vogt, Volker; Post, Carol; Rossmann, Michael. "Structure and Self-association of the Rous Sarcoma Virus Capsid Protein" Structure Fold Des. 8, 617-628 (2000).
Assembly members:
RSV_CA, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Virus Kingdom: not available Genus/species: Avian type C retroviruses Rous sarcoma virus - Prague C strain
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3XC (NOVAGEN)
Entity Sequences (FASTA):
RSV_CA: MDIMQGPSESFVDFANRLIK
AVEGSDLPPSARAPVIIDCF
RQKSQPDIQQLIRTAPSTLT
TPGEIIKYVLDRQKTAPLTD
QGIAAAMSSAIQPLIM
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 554 |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 80 |
| coupling constants | 122 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RSV CA | 1 |
Entities:
Entity 1, RSV CA 96 residues - Formula weight is not available
| 1 | MET | ASP | ILE | MET | GLN | GLY | PRO | SER | GLU | SER | ||||
| 2 | PHE | VAL | ASP | PHE | ALA | ASN | ARG | LEU | ILE | LYS | ||||
| 3 | ALA | VAL | GLU | GLY | SER | ASP | LEU | PRO | PRO | SER | ||||
| 4 | ALA | ARG | ALA | PRO | VAL | ILE | ILE | ASP | CYS | PHE | ||||
| 5 | ARG | GLN | LYS | SER | GLN | PRO | ASP | ILE | GLN | GLN | ||||
| 6 | LEU | ILE | ARG | THR | ALA | PRO | SER | THR | LEU | THR | ||||
| 7 | THR | PRO | GLY | GLU | ILE | ILE | LYS | TYR | VAL | LEU | ||||
| 8 | ASP | ARG | GLN | LYS | THR | ALA | PRO | LEU | THR | ASP | ||||
| 9 | GLN | GLY | ILE | ALA | ALA | ALA | MET | SER | SER | ALA | ||||
| 10 | ILE | GLN | PRO | LEU | ILE | MET |
Samples:
sample_1: RSV_CA, [U-15N], 1.5 mM; Sodium phosphate 50 mM; NaCl 50 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_2: RSV_CA, [U-15N; U-13C], 1.5 mM; Sodium phosphate 50 mM; NaCl 50 mM; EDTA 1 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 4.9; temperature: 298 K; ionic strength: 0.1 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
Software:
NMRPipe v1.6 - data processing
ANSIG v3.3 - data analysis
CNS v1.0 - refinement
NMR spectrometers:
- Varian UnityPlus 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA24512 CAA36153 CAA48534 |
| GB | AAA66157 AAB59932 AAB59933 AAC82560 AAC82561 |
| REF | NP_056886 NP_056887 NP_955609 |
| SP | P03322 P03354 |
| AlphaFold | P03322 P03354 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks