BMRB Entry 4578

Title:
1H, 13C and 15N NMR Assignment of the Hyperstable Quintuple Mutant of Pseudomonas aeruginosa Cytochrome c-551.
Deposition date:
2000-01-28
Original release date:
2000-11-29
Authors:
Hasegawa, Jun; Sambongi, Yoshihiro; Kobayashi, Yuji; Igarashi, Yasuo
Citation:

Citation: Hasegawa, Jun; Uchiyama, Susumu; Yuko, Tanimoto; Masayuki, Mizutani; Yuji, Kobayashi; Yoshihiro, Sambongi; Igarashi, Yasuo. "Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart"  J. Biol. Chem. 275, 37824-37828 (2000).
PubMed: 10918067

Assembly members:

Assembly members:
Psedomonas aeruginosa cytochrome c-551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Psedomonas aeruginosa cytochrome c-551: EDPEVLAKNKGCMACHAIDT KMVGPAYKDVAAKYAGQAGA EAYLAQRIKNGSQGVWGPIP MPPNAVSDDEAQTLAKWILS QK

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts88
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cyt c5511
2heme c2

Entities:

Entity 1, Cyt c551 82 residues - Formula weight is not available

1   GLUASPPROGLUVALLEUALALYSASNLYS
2   GLYCYSMETALACYSHISALAILEASPTHR
3   LYSMETVALGLYPROALATYRLYSASPVAL
4   ALAALALYSTYRALAGLYGLNALAGLYALA
5   GLUALATYRLEUALAGLNARGILELYSASN
6   GLYSERGLNGLYVALTRPGLYPROILEPRO
7   METPROPROASNALAVALSERASPASPGLU
8   ALAGLNTHRLEUALALYSTRPILELEUSER
9   GLNLYS

Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: Psedomonas aeruginosa cytochrome c-551, [U-95% 13C; U-95% 15N], 1.0 mM

Ex-cond_1: pH: 5.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availableEx-cond_1
2D DQF-COSYsample_1not availableEx-cond_1
2D 1H-15N HSQCsample_1not availableEx-cond_1
2D 1H-13C CT-HSQCsample_1not availableEx-cond_1
3D 15N edited NOESY-HSQCsample_1not availableEx-cond_1
3D HNHAsample_1not availableEx-cond_1
3D HNHBsample_1not availableEx-cond_1
3D HNCACBsample_1not availableEx-cond_1
3D CBCA(CO)NNHsample_1not availableEx-cond_1
3D (HB)CBCACO(CA)HAsample_1not availableEx-cond_1
3D HNCOsample_1not availableEx-cond_1
3D HCCH-TOCSYsample_1not availableEx-cond_1
3D 13C,15N edited NOESY-HSQCsample_1not availableEx-cond_1

Software:

PIPP -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks