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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4577
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Markus, Michelle; Gerstner, Resi; Draper, David; Torchia, Dennis. "The solution structure of ribosomal protein S4 delta41 reveals two subdomains
and a positively charged surface that may interact with RNA." EMBO J. 17, 4559-4571 (1998).
Assembly members:
RIBOSOMAL PROTEIN S4 DELTA 41, polymer, 159 residues, 18595 Da.
Natural source: Common Name: Bacteria Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET13A
Entity Sequences (FASTA):
RIBOSOMAL PROTEIN S4 DELTA 41: MKLSEYGLQLQEKQKLRHMY
GVNERQFRKTFEEAGKMPGK
HGENFMILLESRLDNLVYRL
GLARTRRQARQLVTHGHILV
DGSRVNIPSYRVKPGQTIAV
REKSRNLQVIKEALEANNYI
PDYLSFDPEKMEGTYTRLPE
RSELPAEINEALIVEFYSR
Data type | Count |
1H chemical shifts | 1080 |
13C chemical shifts | 712 |
15N chemical shifts | 173 |
coupling constants | 113 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | S4D41 | 1 |
Entity 1, S4D41 159 residues - 18595 Da.
1 | MET | LYS | LEU | SER | GLU | TYR | GLY | LEU | GLN | LEU | ||||
2 | GLN | GLU | LYS | GLN | LYS | LEU | ARG | HIS | MET | TYR | ||||
3 | GLY | VAL | ASN | GLU | ARG | GLN | PHE | ARG | LYS | THR | ||||
4 | PHE | GLU | GLU | ALA | GLY | LYS | MET | PRO | GLY | LYS | ||||
5 | HIS | GLY | GLU | ASN | PHE | MET | ILE | LEU | LEU | GLU | ||||
6 | SER | ARG | LEU | ASP | ASN | LEU | VAL | TYR | ARG | LEU | ||||
7 | GLY | LEU | ALA | ARG | THR | ARG | ARG | GLN | ALA | ARG | ||||
8 | GLN | LEU | VAL | THR | HIS | GLY | HIS | ILE | LEU | VAL | ||||
9 | ASP | GLY | SER | ARG | VAL | ASN | ILE | PRO | SER | TYR | ||||
10 | ARG | VAL | LYS | PRO | GLY | GLN | THR | ILE | ALA | VAL | ||||
11 | ARG | GLU | LYS | SER | ARG | ASN | LEU | GLN | VAL | ILE | ||||
12 | LYS | GLU | ALA | LEU | GLU | ALA | ASN | ASN | TYR | ILE | ||||
13 | PRO | ASP | TYR | LEU | SER | PHE | ASP | PRO | GLU | LYS | ||||
14 | MET | GLU | GLY | THR | TYR | THR | ARG | LEU | PRO | GLU | ||||
15 | ARG | SER | GLU | LEU | PRO | ALA | GLU | ILE | ASN | GLU | ||||
16 | ALA | LEU | ILE | VAL | GLU | PHE | TYR | SER | ARG |
15N_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N], 0.800 mM; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM
15N13C_sample_1: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-13C], 1.1 mM; D2O 6%; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM
15N13C_sample_2: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-13C], 1.1 mM; D2O 99.9%; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM
bicelle_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N], 0.100 mM; 3:1 DMPC:DHPC n/a; KH2PO4 10 mM; sodium azide 0.1 mM; D2O 7%
Pf1_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR, 0.214 mM; Pf1 phage 2.5 mg/ml; KH2PO4 10 mM; KCl 300 mM; sodium azide 0.2 mM; D2O 6%
sample_cond_1: pH: 5.4; temperature: 310 K; pressure: 1 atm
sample_cond_2: pH: 6.1; temperature: 310 K; pressure: 1 atm
sample_cond_3: pH: 6.5; temperature: 310 K; pressure: 1 atm
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCACB | not available | not available | not available |
CBCA(CO)NH | not available | not available | not available |
HNCA | not available | not available | not available |
HCACO | not available | not available | not available |
HNCO | not available | not available | not available |
HBHA(CBCACO)NH | not available | not available | not available |
HNHB | not available | not available | not available |
C(CO)NH | not available | not available | not available |
H(CCO)NH | not available | not available | not available |
HCCH-TOCSY | not available | not available | not available |
13C HSQC | not available | not available | not available |
3D_15N-separated_NOESY | not available | not available | not available |
3D_13C-separated_NOESY | not available | not available | not available |
4D_13C-separated_NOESY | not available | not available | not available |
HNHA | not available | not available | not available |
IPAP 15N HSQC | not available | not available | not available |
XWINNMR v2.5 - spectrometer operation and preliminary processing
NMRPIPE v1.7 - processing spectra
PIPP v4.2.8 - spectral analysis
X-PLOR v3.8, 4.0 - solving the structure
PDB | |
DBJ | BAD77087 GAD11883 GAJ57797 |
GB | AAB21091 ACX77386 ADI25738 ADU95212 AEV20455 |
REF | WP_011232276 WP_013144461 WP_025949527 WP_031407624 WP_033014494 |
SP | P81288 Q5KW49 |
AlphaFold | P81288 Q5KW49 |
Download HSQC peak lists in one of the following formats:
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