BMRB Entry 4572
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4572
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Citation: Sprules, Tara; Green, Nancy; Featherstone, Mark; Gehring, Kalle. "Conformational changes in the PBX Homeodomain and C-terminal Extension upon
Binding DNA and HOX-derived YPWM Peptides" Biochemistry 39, 9943-9950 (2000).
Assembly members:
PBX1 homeodomain, polymer, 75 residues, 8874 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: not available Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b
Entity Sequences (FASTA):
PBX1 homeodomain: GHHHHHHHHHHSSGHIEGRH
MNKQATEILNEYFYSHLSNP
YPSEEAKEELAKKCGITVSQ
VSNWFGNKRIRYKKN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 448 |
| 13C chemical shifts | 244 |
| 15N chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PBX HD | 1 |
Entities:
Entity 1, PBX HD 75 residues - 8874 Da.
| 1 | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | SER | SER | GLY | HIS | ILE | GLU | GLY | ARG | HIS | ||||
| 3 | MET | ASN | LYS | GLN | ALA | THR | GLU | ILE | LEU | ASN | ||||
| 4 | GLU | TYR | PHE | TYR | SER | HIS | LEU | SER | ASN | PRO | ||||
| 5 | TYR | PRO | SER | GLU | GLU | ALA | LYS | GLU | GLU | LEU | ||||
| 6 | ALA | LYS | LYS | CYS | GLY | ILE | THR | VAL | SER | GLN | ||||
| 7 | VAL | SER | ASN | TRP | PHE | GLY | ASN | LYS | ARG | ILE | ||||
| 8 | ARG | TYR | LYS | LYS | ASN |
Samples:
sample_1: PBX1 homeodomain, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM
condition_1: pH: 4.8; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HMQC NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H COSY | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| TOCSY(CO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks