BMRB Entry 4559

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4559

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Title:
The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle
Deposition date:
1999-12-13
Original release date:
2000-10-06
Authors:
Klein, Daniel; Johnson, Philip; Zollars, Eric; De Guzman, Roberto; Summers, Michael
Citation:

Citation: Klein, Daniel; Johnson, Philip; Zollars, Eric; De Guzman, Roberto; Summers, Michael. "The NMR structure of the nucleocapsid protein from the mouse mammary tumor virus reveals unusual folding of the C-terminal zinc knuckle"  Biochemistry 39, 1604-1612 (2000).
PubMed: 10677209

Assembly members:

Assembly members:
mouse mammary tumor virus nucleocapsid protein, polymer, 60 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Mammalian type B retroviruses   Taxonomy ID: 11633   Superkingdom: not available   Kingdom: not available   Genus/species: Mammalian type B retroviruses not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mouse mammary tumor virus nucleocapsid protein: PVCFSCGKTGHIKRDCKEEK GSKRAPPGLCPRCKKGYHWK SECKSKFDKDGNPLPPLETN

Data sets:
Data typeCount
1H chemical shifts248
15N chemical shifts42

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mmtv nc1
2zinc ion 12
3zinc ion 22

Entities:

Entity 1, mmtv nc 60 residues - Formula weight is not available

1   PROVALCYSPHESERCYSGLYLYSTHRGLY
2   HISILELYSARGASPCYSLYSGLUGLULYS
3   GLYSERLYSARGALAPROPROGLYLEUCYS
4   PROARGCYSLYSLYSGLYTYRHISTRPLYS
5   SERGLUCYSLYSSERLYSPHEASPLYSASP
6   GLYASNPROLEUPROPROLEUGLUTHRASN

Entity 2, zinc ion 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: mouse mammary tumor virus nucleocapsid protein, [U-15N], mM; acetate, [U-2H], 25 mM; NaCl 25 mM; ZnCl2 0.1 M; BME 0.1 mM

sample_2: mouse mammary tumor virus nucleocapsid protein mM; acetate, [U-2H], 25 mM; NaCl 25 mM; ZnCl2 0.1 M; BME 0.1 mM

sample_cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESY (50 and 200 ms)not availablenot availablesample_cond_1
15N-edited NOESY-HSQC (m = 200 ms)not availablenot availablesample_cond_1
15N,15N-edited HMQC-NOESY-HSQC (m = 120 ms)not availablenot availablesample_cond_1
15N-edited TOCSY 70 ms clean-MLEV-17 mixing periodnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
HNHBnot availablenot availablesample_cond_1
15N T1not availablenot availablesample_cond_1
15N T2not availablenot availablesample_cond_1
15N-{1H} NOEnot availablenot availablesample_cond_1

Software:

DYANA - structure calculation

NMR spectrometers:

  • Bruker DMX 600 MHz
  • GE Omega PSG 600 MHz

Related Database Links:

PDB
DBJ BAA03765
GB AAA66623 AAF31472 AAF31473 AAF31474
SP P11283 P11284 Q9IZT2
AlphaFold P11283 P11284 Q9IZT2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks