BMRB Entry 4556

Title:
31P NMR analysis of the DNA conformation induced by protein binding:SRY-DNA complexes
Deposition date:
1999-12-11
Original release date:
2000-01-11
Authors:
Castagne, Claire; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel
Citation:

Citation: Castagne, Claire; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel. "31P NMR analysis of the DNA conformation induced by protein binding:SRY-DNA complexes"  Eur. J. Biochem. ., .-..

Assembly members:

Assembly members:
SRY 8mer strand 1, polymer, 8 residues, Formula weight is not available
SRY 8mer strand 2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: na na

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SRY 8mer strand 1: GCACAAAC
SRY 8mer strand 2: GTTTGTGC

Data sets:
Data typeCount
1H chemical shifts137
31P chemical shifts14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
18mer strand 11
28mer strand 22

Entities:

Entity 1, 8mer strand 1 8 residues - Formula weight is not available

1   DGDCDADCDADADADC

Entity 2, 8mer strand 2 8 residues - Formula weight is not available

1   DGDTDTDTDGDTDGDC

Samples:

SRY-8mer: SRY 8mer strand 1 2.5 mM; SRY 8mer strand 2 2.5 mM

cond1: pH: 7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H COSYSRY-8mernot availablecond1
1H-1H TOCSYSRY-8mernot availablecond1
1H-1H NOESYSRY-8mernot availablecond1
1H-31P HSQC-TOCSYSRY-8mernot availablecond1
1H-31P HCOSYINVSRY-8mernot availablecond1
31P-31P NOESY ExchangeSRY-8mernot availablecond1

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz