BMRB Entry 4522
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4522
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Citation: Wang, Y.; Neamati, N.; Jacob, J.; Palmer, I.; Stahl, S.. "Solution Structure of Anti-HIV-1 and Anti-Tumor Protein Map30: Structural
Insights Into its Multiple Functions " Cell 99, 433-442 (1999).
Assembly members:
MAP30, polymer, 263 residues, Formula weight is not available
Natural source: Common Name: balsam pear Taxonomy ID: 3673 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Momordica charantia
Experimental source: Production method: .
Entity Sequences (FASTA):
MAP30: DVNFDLSTATAKTYTKFIED
FRATLPFSHKVYDIPLLYST
ISDSRRFILLNLTSYAYETI
SVAIDVTNVYVVAYRTRDVS
YFFKESPPEAYNILFKGTRK
ITLPYTGNYENLQTAAHKIR
ENIDLGLPALSSAITTLFYY
NAQSAPSALLVLIQTTAEAA
RFKYIERHVAKYVATNFKPN
LAIISLENQWSALSKQIFLA
QNQGGKFRNPVDLIKPTGER
FQVTNVDSDVVKGNIKLLLN
SRASTADENFITTMTLLGES
VVN
| Data type | Count |
| residual dipolar couplings | 387 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MAP30 | 1 |
Entities:
Entity 1, MAP30 263 residues - Formula weight is not available
| 1 | ASP | VAL | ASN | PHE | ASP | LEU | SER | THR | ALA | THR | ||||
| 2 | ALA | LYS | THR | TYR | THR | LYS | PHE | ILE | GLU | ASP | ||||
| 3 | PHE | ARG | ALA | THR | LEU | PRO | PHE | SER | HIS | LYS | ||||
| 4 | VAL | TYR | ASP | ILE | PRO | LEU | LEU | TYR | SER | THR | ||||
| 5 | ILE | SER | ASP | SER | ARG | ARG | PHE | ILE | LEU | LEU | ||||
| 6 | ASN | LEU | THR | SER | TYR | ALA | TYR | GLU | THR | ILE | ||||
| 7 | SER | VAL | ALA | ILE | ASP | VAL | THR | ASN | VAL | TYR | ||||
| 8 | VAL | VAL | ALA | TYR | ARG | THR | ARG | ASP | VAL | SER | ||||
| 9 | TYR | PHE | PHE | LYS | GLU | SER | PRO | PRO | GLU | ALA | ||||
| 10 | TYR | ASN | ILE | LEU | PHE | LYS | GLY | THR | ARG | LYS | ||||
| 11 | ILE | THR | LEU | PRO | TYR | THR | GLY | ASN | TYR | GLU | ||||
| 12 | ASN | LEU | GLN | THR | ALA | ALA | HIS | LYS | ILE | ARG | ||||
| 13 | GLU | ASN | ILE | ASP | LEU | GLY | LEU | PRO | ALA | LEU | ||||
| 14 | SER | SER | ALA | ILE | THR | THR | LEU | PHE | TYR | TYR | ||||
| 15 | ASN | ALA | GLN | SER | ALA | PRO | SER | ALA | LEU | LEU | ||||
| 16 | VAL | LEU | ILE | GLN | THR | THR | ALA | GLU | ALA | ALA | ||||
| 17 | ARG | PHE | LYS | TYR | ILE | GLU | ARG | HIS | VAL | ALA | ||||
| 18 | LYS | TYR | VAL | ALA | THR | ASN | PHE | LYS | PRO | ASN | ||||
| 19 | LEU | ALA | ILE | ILE | SER | LEU | GLU | ASN | GLN | TRP | ||||
| 20 | SER | ALA | LEU | SER | LYS | GLN | ILE | PHE | LEU | ALA | ||||
| 21 | GLN | ASN | GLN | GLY | GLY | LYS | PHE | ARG | ASN | PRO | ||||
| 22 | VAL | ASP | LEU | ILE | LYS | PRO | THR | GLY | GLU | ARG | ||||
| 23 | PHE | GLN | VAL | THR | ASN | VAL | ASP | SER | ASP | VAL | ||||
| 24 | VAL | LYS | GLY | ASN | ILE | LYS | LEU | LEU | LEU | ASN | ||||
| 25 | SER | ARG | ALA | SER | THR | ALA | ASP | GLU | ASN | PHE | ||||
| 26 | ILE | THR | THR | MET | THR | LEU | LEU | GLY | GLU | SER | ||||
| 27 | VAL | VAL | ASN |
Samples:
sample_1: MAP30, [U-15N; U-13C], 0.7 mM
sample_cond_1: pH: 5.50; temperature: 313.00 K; ionic strength: 0.01 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C/15N-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 3D 13C-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| 4D 13C-SEPARATED NOESY | sample_1 | not available | sample_cond_1 |
| HNHA | sample_1 | not available | sample_cond_1 |
| HNCA-J | sample_1 | not available | sample_cond_1 |
Software:
MODIFIED XPLOR v3.5 - refinement, structure solution
NMR spectrometers:
- BRUKER DMX 750 MHz