BMRB Entry 4493

Title:
Solution structure of the designed hydrophobic core mutant of ubiquitin, 1D7
Deposition date:
1999-04-08
Original release date:
2000-06-16
Authors:
Johnson, E.; Lazar, G.; Desjarlais, J.; Handel, T.
Citation:

Citation: Johnson, E.; Lazar, G.; Desjarlais, J.; Handel, T.. "Solution structure and dynamics of a designed hydrophobic core variant of ubiquitin"  Structure 7, 967-976 (1999).
PubMed: 10467150

Assembly members:

Assembly members:
Ubiquitin core mutant 1D7, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubiquitin core mutant 1D7: MQVFLKTLTGKTVTIEVEPS DTVENFKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTIHLVLRLRGG

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts84
1H chemical shifts562

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubiquitin core mutant 1D71

Entities:

Entity 1, Ubiquitin core mutant 1D7 76 residues - Formula weight is not available

1   METGLNVALPHELEULYSTHRLEUTHRGLY
2   LYSTHRVALTHRILEGLUVALGLUPROSER
3   ASPTHRVALGLUASNPHELYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRILEHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: Ubiquitin core mutant 1D7, [U-13C; U-15N], 2 mM; sodium acetate D3 25 mM; sodium azide 0.02%

sample_cond_1: pH: 5.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N edited 3D NOESYnot availablenot availablenot available
13C edited 3D NOESYnot availablenot availablenot available
13C/13C edited 4D noesynot availablenot availablenot available
3D 3Jhnhanot availablenot availablenot available
3D hacahbnot availablenot availablenot available
2D 3Jncgnot availablenot availablenot available
2d 3Jcocgnot availablenot availablenot available
long range 3Jccnot availablenot availablenot available
3D 15N ROESYnot availablenot availablenot available
3D 15N TOCSYnot availablenot availablenot available
1Dnh residual dipolar couplingsnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAB79488
EMBL CAA25706 CAD91438 CAG28691 CAK80500 CDJ67220
GB AAA36787 AAM46896 AAO66467 AAV84266 ABC94632
PRF 1101405A
REF XP_001447897 XP_001621345 XP_004595836 XP_005908561 XP_007603155

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks