BMRB Entry 4493
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4493
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Johnson, E.; Lazar, G.; Desjarlais, J.; Handel, T.. "Solution structure and dynamics of a designed hydrophobic core variant of
ubiquitin" Structure 7, 967-976 (1999).
PubMed: 10467150
Assembly members:
Ubiquitin core mutant 1D7, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Ubiquitin core mutant 1D7: MQVFLKTLTGKTVTIEVEPS
DTVENFKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
IQKESTIHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 273 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 562 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin core mutant 1D7 | 1 |
Entities:
Entity 1, Ubiquitin core mutant 1D7 76 residues - Formula weight is not available
| 1 | MET | GLN | VAL | PHE | LEU | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | VAL | THR | ILE | GLU | VAL | GLU | PRO | SER | ||||
| 3 | ASP | THR | VAL | GLU | ASN | PHE | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | ILE | GLN | LYS | GLU | SER | THR | ILE | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin core mutant 1D7, [U-13C; U-15N], 2 mM; sodium acetate D3 25 mM; sodium azide 0.02%
sample_cond_1: pH: 5.8; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N edited 3D NOESY | not available | not available | not available |
| 13C edited 3D NOESY | not available | not available | not available |
| 13C/13C edited 4D noesy | not available | not available | not available |
| 3D 3Jhnha | not available | not available | not available |
| 3D hacahb | not available | not available | not available |
| 2D 3Jncg | not available | not available | not available |
| 2d 3Jcocg | not available | not available | not available |
| long range 3Jcc | not available | not available | not available |
| 3D 15N ROESY | not available | not available | not available |
| 3D 15N TOCSY | not available | not available | not available |
| 1Dnh residual dipolar couplings | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB79488 |
| EMBL | CAA25706 CAD91438 CAG28691 CAK80500 CDJ67220 |
| GB | AAA36787 AAM46896 AAO66467 AAV84266 ABC94632 |
| PRF | 1101405A |
| REF | XP_001447897 XP_001621345 XP_004595836 XP_005908561 XP_007603155 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks