BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4470

Title: Solution Structure of ThiS and Implications for the Evolutionary Roots of Ubiquitin

Deposition date: 1999-12-02 Original release date: 2001-02-15

Authors: Wang, Chunyu; Xi, Jun; Begley, Tadhg; Nicholson, Linda

Citation: Wang, Chunyu; Xi, Jun; Begley, Tadhg; Nicholson, Linda. "Solution Structure of ThiS and Implications for the Evolutionary Roots of Ubiquitin"  Nat. Struct. Biol. 8, 47-51 (2001).

Assembly members:
ThiS, polymer, 66 residues, Formula weight is not available

Natural source:   Common Name: Escherishia Coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherishia Coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ThiS: MQILFNDQAMQCAAGQTVHE LLEQLDQRQAGAALAINQQI VPREQWAQHIVQDGDQILLF QVIAGG

Data sets:
Data typeCount
1H chemical shifts376
13C chemical shifts186
15N chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ThiS monomer1

Entities:

Entity 1, ThiS monomer 66 residues - Formula weight is not available

1   METGLNILELEUPHEASNASPGLNALAMET
2   GLNCYSALAALAGLYGLNTHRVALHISGLU
3   LEULEUGLUGLNLEUASPGLNARGGLNALA
4   GLYALAALALEUALAILEASNGLNGLNILE
5   VALPROARGGLUGLNTRPALAGLNHISILE
6   VALGLNASPGLYASPGLNILELEULEUPHE
7   GLNVALILEALAGLYGLY

Samples:

sample_1: ThiS, [U-95% 15N], 0.8 mM

sample_2: ThiS, [U-95% 15N; U-95% 13C], 1.0 mM

cond_1: pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCA, C(CO)Nnot availablenot availablenot available
H(CCO)Nnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

nmrPipe -

PIPP -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE77328 BAG79803 BAI28252 BAI33435 BAI38553
EMBL CAP78447 CAQ34338 CAR00965 CAR05621 CAR15639
GB AAB95620 AAT48237 AAZ90669 ABE09257 ABF06055
REF WP_001166226 WP_001166228 WP_001166229 WP_001166230 WP_001166232
SP O32583
AlphaFold O32583

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts