BMRB Entry 4467

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PDB ID: 1ub1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4467
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 13C, and 15N Assignments of the MAR-binding Domain of Chicken MeCP2/ARBP

Deposition date:

1999-12-01

Original release date:

2002-07-12

Authors:

Brunner, Eike; Weitzel, Joachim; Heitmann, Bjoern; Maurer, Till; Straetling, Wolf; Kalbitzer, Hans Robert

Citation:

Citation: Brunner, Eike; Weitzel, Joachim; Heitmann, Bjoern; Maurer, Till; Straetling, Wolf; Kalbitzer, Hans Robert. "Sequence-specific 1H, 13C, and 15N Assignments of the MAR-binding Domain of Chicken MeCP2/ARBP" J. Biomol. NMR 17, 175-176 (2000).

Assembly members:

Assembly members:
Methyl-CpG-binding protein, attachment region binding protein, polymer, 125 residues, 16300 Da.

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-cARBP-Ex4.2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Methyl-CpG-binding protein, attachment region binding protein: APAVPEASASPKQRRSIIRD RGPMYDDPTLPEGWTRKLKQ RKSGRSAGKYDVYLINPQGK AFRSKVELIAYFEKVGDTSL DPNDFDFTVTGRGSPSRREQ RPPKKAKSPKSPGSGRGRGR PKGSG

Data sets:

assigned_chemical_shifts
- Assigned_chemical_shifts_set_1

Data type	Count
1H chemical shifts	231
13C chemical shifts	319
15N chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MeCP2/ARBP	1

Entities:

Entity 1, MeCP2/ARBP 125 residues - 16300 Da.

1

ALA

PRO

ALA

VAL

PRO

GLU

ALA

SER

ALA

SER

2

PRO

LYS

GLN

ARG

SER

ILE

ARG

ASP

3

ARG

GLY

PRO

MET

TYR

ASP

PRO

THR

LEU

4

PRO

GLU

GLY

TRP

THR

ARG

LYS

LEU

LYS

GLN

5

ARG

LYS

SER

GLY

ARG

SER

ALA

GLY

LYS

TYR

6

ASP

VAL

TYR

LEU

ILE

ASN

PRO

GLN

GLY

LYS

7

ALA

PHE

ARG

SER

LYS

VAL

GLU

LEU

ILE

ALA

8

TYR

PHE

GLU

LYS

VAL

GLY

ASP

THR

SER

LEU

9

ASP

PRO

ASN

ASP

PHE

ASP

PHE

THR

VAL

THR

10

GLY

ARG

GLY

SER

PRO

SER

ARG

GLU

GLN

11

ARG

PRO

LYS

ALA

LYS

SER

PRO

LYS

12

SER

PRO

GLY

SER

GLY

ARG

GLY

ARG

GLY

ARG

13

PRO

LYS

GLY

SER

GLY

Samples:

sample_1: Methyl-CpG-binding protein, attachment region binding protein, [U-15N; U-13C], 2.0 mM

sample_2: Methyl-CpG-binding protein, attachment region binding protein, [U-15N], 2.0 mM

sample_3: Methyl-CpG-binding protein, attachment region binding protein 2.0 mM

sample_4: Methyl-CpG-binding protein, attachment region binding protein, [U-2H; U-13C; U-15N], 2.0 mM

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	Ex-cond_1
CBCA(CO)NH	not available	not available	Ex-cond_1
1H-15N-HSQC	not available	not available	Ex-cond_1
1H-15N-NOESY HSQC	not available	not available	Ex-cond_1
1H-15N-TOCSY HSQC	not available	not available	Ex-cond_1
HNCO	not available	not available	Ex-cond_1
HCCH-TOCSY	not available	not available	Ex-cond_1

Software:

AURELIA v2.5 - visualisation

XWINNMR v2.5 - processing

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

BMRB	4280
PDB	1QK9 1UB1
EMBL	CAA74577
GB	EPY78157
REF	XP_004645154 XP_004645155 XP_006113271 XP_007054114 XP_008175578