BMRB Entry 4462

Title:
1H Chemical Shift Assignements for mouse [Cd7]-metallothionein-1
Deposition date:
1999-11-21
Original release date:
2000-03-24
Authors:
Zangger, Klaus; Oz, Gulin; Otvos, James; Armitage, Ian
Citation:

Citation: Zangger, Klaus; Oz, Gulin; Otvos, James; Armitage, Ian. "Three-dimensional solution structure of mouse [Cd7]-metallothionein-1 by homonuclear and heteronuclear NMR spectroscopy"  Protein Sci. 8, 2630-2638 (1999).
PubMed: 10631978

Assembly members:

Assembly members:
mouse [Cd7]-metallothionein-1, polymer, 61 residues, Formula weight is not available
CADMIUM ION, non-polymer, 112.411 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mouse [Cd7]-metallothionein-1: MDPNCSCSTGGSCTCTSSCA CKNCKCTSCKKSCCSCCPVG CSKCAQGCVCKGAADKCTCC A

Data sets:
Data typeCount
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse MT11
2113Cd 12
3113Cd 22
4113Cd 32
5113Cd 42
6113Cd 52
7113Cd 62
8113Cd 72

Entities:

Entity 1, mouse MT1 61 residues - Formula weight is not available

1   METASPPROASNCYSSERCYSSERTHRGLY
2   GLYSERCYSTHRCYSTHRSERSERCYSALA
3   CYSLYSASNCYSLYSCYSTHRSERCYSLYS
4   LYSSERCYSCYSSERCYSCYSPROVALGLY
5   CYSSERLYSCYSALAGLNGLYCYSVALCYS
6   LYSGLYALAALAASPLYSCYSTHRCYSCYS
7   ALA

Entity 2, 113Cd 1 - Cd - 112.411 Da.

1   CD

Samples:

sample_1: mouse [Cd7]-metallothionein-1 0.7 mM

Experimental_conditions_1: pH: 6.5; temperature: 273 K; ionic strength: 0.18 M

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availableExperimental_conditions_1
2D TOCSYsample_1not availableExperimental_conditions_1
Cd-H accordion HMQCsample_1not availableExperimental_conditions_1
The NOESY and TOCSY sequences had a watergate block at the end for solventsample_1not availableExperimental_conditions_1
suppressionsample_1not availableExperimental_conditions_1

Software:

VNMR v6.1a - spectral processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAC25563
GB AAA39527 AAB26768 AAH36990 EDL11119
PIR I54574
REF NP_038630
SP P02802
AlphaFold P02802