BMRB Entry 4449

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Intramolecular Dimer Antifreeze Protein RD
Deposition date:
1999-11-02
Original release date:
2000-03-22
Authors:
Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae
Citation:

Citation: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignments of 1H, 13C, and 15N resonances of intramolecular dimer antifreeze protein RD3"  J. Biomol. NMR 16, 273-274 (2000).

Assembly members:

Assembly members:
antifreeze protein type III, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Antarctic eelpout   Taxonomy ID: 8195   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Austrolycichthys brachycephalus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pkk223-3UC

Data sets:
Data typeCount
13C chemical shifts259
15N chemical shifts140
1H chemical shifts973
coupling constants80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RD31

Entities:

Entity 1, RD3 134 residues - Formula weight is not available

1   ASNLYSALASERVALVALALAASNGLNLEU
2   ILEPROILEASNTHRALALEUTHRLEUILE
3   METMETLYSALAGLUVALVALTHRPROMET
4   GLYILEPROALAGLUGLUILEPROASNLEU
5   VALGLYMETGLNVALASNARGALAVALPRO
6   LEUGLYTHRTHRLEUMETPROASPMETVAL
7   LYSASNTYRGLUASPGLYTHRTHRSERPRO
8   GLYLEULYSSERVALVALALAASNGLNLEU
9   ILEPROILEASNTHRALALEUTHRLEUVAL
10   METMETLYSALAGLUGLUVALSERPROLYS
11   GLYILEPROSERGLUGLUILESERLYSLEU
12   VALGLYMETGLNVALASNARGALAVALTYR
13   LEUASPGLNTHRLEUMETPROASPMETVAL
14   LYSASNTYRGLU

Samples:

sample_1: antifreeze protein type III 1.0 mM

sample_2: antifreeze protein type III, [U-90% 15N], 1.0 mM

sample_3: antifreeze protein type III, [U-95% 13C; U-90% 15N], 1.0 mM

Ex-cond_1: ionic strength: 0.025 M; pH: 6.8; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availableEx-cond_1
1H-15N TOCSYnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
C(CO)NHnot availablenot availableEx-cond_1
HC(CO)NHnot availablenot availableEx-cond_1

Software:

NMRPipe v1.6 - data processing

PIPP v4.0.4 - assignment

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 4450
PDB
PRF 2109220B
SP P35753
AlphaFold P35753

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks