BMRB Entry 4449

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4449

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Intramolecular Dimer Antifreeze Protein RD

Deposition date:

1999-11-02

Original release date:

2000-03-22

Authors:

Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae

Citation:

Citation: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignments of 1H, 13C, and 15N resonances of intramolecular dimer antifreeze protein RD3" J. Biomol. NMR 16, 273-274 (2000).

Assembly members:

Assembly members:
antifreeze protein type III, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Antarctic eelpout Taxonomy ID: 8195 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Austrolycichthys brachycephalus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pkk223-3UC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
antifreeze protein type III: NKASVVANQLIPINTALTLI MMKAEVVTPMGIPAEEIPNL VGMQVNRAVPLGTTLMPDMV KNYEDGTTSPGLKSVVANQL IPINTALTLVMMKAEEVSPK GIPSEEISKLVGMQVNRAVY LDQTLMPDMVKNYE

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
13C chemical shifts	259
15N chemical shifts	140
1H chemical shifts	973
coupling constants	80

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RD3	1

Entities:

Entity 1, RD3 134 residues - Formula weight is not available

1

ASN

LYS

ALA

SER

VAL

ALA

ASN

GLN

LEU

2

ILE

PRO

ILE

ASN

THR

ALA

LEU

THR

LEU

ILE

3

MET

LYS

ALA

GLU

VAL

THR

PRO

MET

4

GLY

ILE

PRO

ALA

GLU

ILE

PRO

ASN

LEU

5

VAL

GLY

MET

GLN

VAL

ASN

ARG

ALA

VAL

PRO

6

LEU

GLY

THR

LEU

MET

PRO

ASP

MET

VAL

7

LYS

ASN

TYR

GLU

ASP

GLY

THR

SER

PRO

8

GLY

LEU

LYS

SER

VAL

ALA

ASN

GLN

LEU

9

ILE

PRO

ILE

ASN

THR

ALA

LEU

THR

LEU

VAL

10

MET

LYS

ALA

GLU

VAL

SER

PRO

LYS

11

GLY

ILE

PRO

SER

GLU

ILE

SER

LYS

LEU

12

VAL

GLY

MET

GLN

VAL

ASN

ARG

ALA

VAL

TYR

13

LEU

ASP

GLN

THR

LEU

MET

PRO

ASP

MET

VAL

14

LYS

ASN

TYR

GLU

Samples:

sample_1: antifreeze protein type III 1.0 mM

sample_2: antifreeze protein type III, [U-90% 15N], 1.0 mM

sample_3: antifreeze protein type III, [U-95% 13C; U-90% 15N], 1.0 mM

Ex-cond_1: ionic strength: 0.025 M; pH: 6.8; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	Ex-cond_1
1H-15N TOCSY	not available	not available	Ex-cond_1
HNCA	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
CBCA(CO)NH	not available	not available	Ex-cond_1
HCCH-TOCSY	not available	not available	Ex-cond_1
C(CO)NH	not available	not available	Ex-cond_1
HC(CO)NH	not available	not available	Ex-cond_1

Software:

NMRPipe v1.6 - data processing

PIPP v4.0.4 - assignment

NMR spectrometers:

Varian Unity 500 MHz

BMRB	4450
PDB	1C89 1C8A
PRF	2109220B
SP	P35753
AlphaFold	P35753