BMRB Entry 4446

Title:
NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius: possible determinants of protein stability.
Deposition date:
1999-10-25
Original release date:
2000-03-10
Authors:
Nicastro, Giuseppe; de Chiara, Cesira
Citation:

Citation: Nicastro, Giuseppe; de Chiara, Cesira. "NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius: possible determinants of protein stability."  Eur. J. Biochem. ., .-..

Assembly members:

Assembly members:
Thioredoxin from Bacillus acidocaldarius, polymer, 105 residues, 11577 Da.

Natural source:

Natural source:   Common Name: Bacillus acidocaldarius   Taxonomy ID: 1388   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Alicyclobacillus acidocaldarius

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: Bacillus acidocaldarius   Taxonomy ID: 1388   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Alicyclobacillus acidocaldarius

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts747

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1thioredoxin1

Entities:

Entity 1, thioredoxin 105 residues - 11577 Da.

1   ALATHRMETTHRLEUTHRASPALAASNPHE
2   GLNGLNALAILEGLNGLYASPLYSPROVAL
3   LEUVALASPPHETRPALAALATRPCYSGLY
4   PROCYSARGMETMETALAPROVALLEUGLU
5   GLUPHEALAGLUALAHISALAASPLYSVAL
6   THRVALALALYSLEUASNVALASPGLUASN
7   PROGLUTHRTHRSERGLNPHEGLYILEMET
8   SERILEPROTHRLEUILELEUPHELYSGLY
9   GLYARGPROVALLYSGLNLEUILEGLYTYR
10   GLNPROLYSGLUGLNLEUGLUALAGLNLEU
11   ALAASPVALLEUGLN

Samples:

sample_1: Thioredoxin from Bacillus acidocaldarius 1.2 mM; phosphate buffer 50 mM

cond_1: ionic strength: 50 mM; pH: 5.8; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
NOESY-JRnot availablenot availablenot available
DQ-2Dnot availablenot availablenot available
MQF-COSYnot availablenot availablenot available
WG-COSYnot availablenot availablenot available
WG-TOCSYnot availablenot availablenot available
WG-NOESYnot availablenot availablenot available
3D-TOCSY-NOESYnot availablenot availablenot available

Software:

FELIX v97 - peaks assignments

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
GB ACV57898 AEJ42820 EED08563
REF WP_008336730 WP_012810252 WP_041695007
SP P80579
AlphaFold P80579