BMRB Entry 4446
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4446
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Citation: Nicastro, Giuseppe; de Chiara, Cesira. "NMR solution structure of a novel thioredoxin from Bacillus acidocaldarius:
possible determinants of protein stability." Eur. J. Biochem. ., .-..
Assembly members:
Thioredoxin from Bacillus acidocaldarius, polymer, 105 residues, 11577 Da.
Natural source: Common Name: Bacillus acidocaldarius Taxonomy ID: 1388 Superkingdom: Eubacteria Kingdom: not available Genus/species: Alicyclobacillus acidocaldarius
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Thioredoxin from Bacillus acidocaldarius: ATMTLTDANFQQAIQGDKPV
LVDFWAAWCGPCRMMAPVLE
EFAEAHADKVTVAKLNVDEN
PETTSQFGIMSIPTLILFKG
GRPVKQLIGYQPKEQLEAQL
ADVLQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 747 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | thioredoxin | 1 |
Entities:
Entity 1, thioredoxin 105 residues - 11577 Da.
| 1 | ALA | THR | MET | THR | LEU | THR | ASP | ALA | ASN | PHE | ||||
| 2 | GLN | GLN | ALA | ILE | GLN | GLY | ASP | LYS | PRO | VAL | ||||
| 3 | LEU | VAL | ASP | PHE | TRP | ALA | ALA | TRP | CYS | GLY | ||||
| 4 | PRO | CYS | ARG | MET | MET | ALA | PRO | VAL | LEU | GLU | ||||
| 5 | GLU | PHE | ALA | GLU | ALA | HIS | ALA | ASP | LYS | VAL | ||||
| 6 | THR | VAL | ALA | LYS | LEU | ASN | VAL | ASP | GLU | ASN | ||||
| 7 | PRO | GLU | THR | THR | SER | GLN | PHE | GLY | ILE | MET | ||||
| 8 | SER | ILE | PRO | THR | LEU | ILE | LEU | PHE | LYS | GLY | ||||
| 9 | GLY | ARG | PRO | VAL | LYS | GLN | LEU | ILE | GLY | TYR | ||||
| 10 | GLN | PRO | LYS | GLU | GLN | LEU | GLU | ALA | GLN | LEU | ||||
| 11 | ALA | ASP | VAL | LEU | GLN |
Samples:
sample_1: Thioredoxin from Bacillus acidocaldarius 1.2 mM; phosphate buffer 50 mM
cond_1: ionic strength: 50 mM; pH: 5.8; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| NOESY-JR | not available | not available | not available |
| DQ-2D | not available | not available | not available |
| MQF-COSY | not available | not available | not available |
| WG-COSY | not available | not available | not available |
| WG-TOCSY | not available | not available | not available |
| WG-NOESY | not available | not available | not available |
| 3D-TOCSY-NOESY | not available | not available | not available |
Software:
FELIX v97 - peaks assignments
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| PDB | |
| GB | ACV57898 AEJ42820 EED08563 |
| REF | WP_008336730 WP_012810252 WP_041695007 |
| SP | P80579 |
| AlphaFold | P80579 |