BMRB Entry 4444

Title:
1H, 13C and 15N chemical shift assignments and secondary structure of Halobacterium salinarum ferredoxin
Deposition date:
1999-10-20
Original release date:
2000-04-03
Authors:
Schweimer, Kristian; Marg, Bianca-Lucia; Oesterhelt, Dieter; Roesch, Paul; Sticht, Heinrich
Citation:

Citation: Schweimer, Kristian; Marg, Bianca-Lucia; Oesterhelt, Dieter; Roesch, Paul; Sticht, Heinrich. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments and secondary structure of [2Fe-2S] ferredoxin from Halobacterium salinarum"  J. Biomol. NMR 16, 347-348 (2000).

Assembly members:

Assembly members:
halobacterium salinarum ferredoxin, polymer, 128 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: Halobacterium salinarum   Taxonomy ID: 2242   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Halobacterium salinarum

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts519
13C chemical shifts349
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HsFdx monomer1
22Fe-2S2

Entities:

Entity 1, HsFdx monomer 128 residues - Formula weight is not available

1   PROTHRVALGLUTYRLEUASNTYRGLUTHR
2   LEUASPASPGLNGLYTRPASPMETASPASP
3   ASPASPLEUPHEGLULYSALAALAASPALA
4   GLYLEUASPGLYGLUASPTYRGLYTHRMET
5   GLUVALALAGLUGLYGLUTYRILELEUGLU
6   ALAALAGLUALAGLNGLYTYRASPTRPPRO
7   PHESERCYSARGALAGLYALACYSALAASN
8   CYSALASERILEVALLYSGLUGLYGLUILE
9   ASPMETASPMETGLNGLNILELEUSERASP
10   GLUGLUVALGLUGLULYSASPVALARGLEU
11   THRCYSILEGLYSERPROALAALAASPGLU
12   VALLYSILEVALTYRASNALAALYHISLEU
13   ASPTYRLEUGLNASNARGVALILE

Entity 2, 2Fe-2S - Fe2 S2 - 175.820 Da.

1   FES

Samples:

sample_1: halobacterium salinarum ferredoxin, [U-99% 13C; U-99% 15N], 0.9 mM

sample_2: halobacterium salinarum ferredoxin, [U-99% 15N], 1.0 mM

sample_3: halobacterium salinarum ferredoxin 0.8 mM

Ex-cond_1: pH: 6.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableEx-cond_1

Software:

NMRView v4.0.1 - spectrum visualization for resonance assignments

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA48224 CAP14708
GB AAG20406
PRF 0407261A 1916400A
REF WP_010903707
SP P00216
AlphaFold P00216

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks