BMRB Entry 4442
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4442
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Citation: Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John. "Interactions in two Prototypical [2Fe-2S] Proteins: Selective (Chiral)
Deuteration and Analysis of 1H and 2H NMR Signals from the Alpha and Beta
Hydrogens of Cysteinyl Residues that Ligate the Iron in the Active Sites of
Human Ferredoxin and Anabaena 712" Arch. Biochem. Biophys. ., .-..
Assembly members:
Anabaena 7120 vegetative Ferredoxin, polymer, 98 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.
Natural source: Common Name: Anabaena 7120 Taxonomy ID: 1174 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anabaena PCC7120
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Anabaena 7120 vegetative Ferredoxin: ATFKVTLINEAEGTKHEIEV
PDDEYILDAAEEQGYDLPFS
CRAGACSTCAGKLVSGTVDQ
SDQSFLDDDQIEAGYVLTCV
AYPTSDVVIQTHKEEDLY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 12 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VFd | 1 |
| 2 | Fe2S2 | 2 |
Entities:
Entity 1, VFd 98 residues - Formula weight is not available
| 1 | ALA | THR | PHE | LYS | VAL | THR | LEU | ILE | ASN | GLU | ||||
| 2 | ALA | GLU | GLY | THR | LYS | HIS | GLU | ILE | GLU | VAL | ||||
| 3 | PRO | ASP | ASP | GLU | TYR | ILE | LEU | ASP | ALA | ALA | ||||
| 4 | GLU | GLU | GLN | GLY | TYR | ASP | LEU | PRO | PHE | SER | ||||
| 5 | CYS | ARG | ALA | GLY | ALA | CYS | SER | THR | CYS | ALA | ||||
| 6 | GLY | LYS | LEU | VAL | SER | GLY | THR | VAL | ASP | GLN | ||||
| 7 | SER | ASP | GLN | SER | PHE | LEU | ASP | ASP | ASP | GLN | ||||
| 8 | ILE | GLU | ALA | GLY | TYR | VAL | LEU | THR | CYS | VAL | ||||
| 9 | ALA | TYR | PRO | THR | SER | ASP | VAL | VAL | ILE | GLN | ||||
| 10 | THR | HIS | LYS | GLU | GLU | ASP | LEU | TYR |
Entity 2, Fe2S2 - Fe2 S2 - 175.820 Da.
| 1 | FES |
Samples:
VFd_reduced: Anabaena 7120 vegetative Ferredoxin2 – 3 mM
sample_conditions: pH*: 8.5 na; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | VFd_reduced | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 400 MHz