BMRB Entry 4442

Title:
Hyperfine Cys Proton Signals from Reduced Anabaena 7120 Vegetative Ferredoxin
Deposition date:
1999-10-15
Original release date:
2000-05-24
Authors:
Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John
Citation:

Citation: Xia, Bin; Jenk, Daniel; LeMaster, David; Westler, William; Markley, John. "Interactions in two Prototypical [2Fe-2S] Proteins: Selective (Chiral) Deuteration and Analysis of 1H and 2H NMR Signals from the Alpha and Beta Hydrogens of Cysteinyl Residues that Ligate the Iron in the Active Sites of Human Ferredoxin and Anabaena 712"  Arch. Biochem. Biophys. ., .-..

Assembly members:

Assembly members:
Anabaena 7120 vegetative Ferredoxin, polymer, 98 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.

Natural source:

Natural source:   Common Name: Anabaena 7120   Taxonomy ID: 1174   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Anabaena PCC7120

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VFd1
2Fe2S22

Entities:

Entity 1, VFd 98 residues - Formula weight is not available

1   ALATHRPHELYSVALTHRLEUILEASNGLU
2   ALAGLUGLYTHRLYSHISGLUILEGLUVAL
3   PROASPASPGLUTYRILELEUASPALAALA
4   GLUGLUGLNGLYTYRASPLEUPROPHESER
5   CYSARGALAGLYALACYSSERTHRCYSALA
6   GLYLYSLEUVALSERGLYTHRVALASPGLN
7   SERASPGLNSERPHELEUASPASPASPGLN
8   ILEGLUALAGLYTYRVALLEUTHRCYSVAL
9   ALATYRPROTHRSERASPVALVALILEGLN
10   THRHISLYSGLUGLUASPLEUTYR

Entity 2, Fe2S2 - Fe2 S2 - 175.820 Da.

1   FES

Samples:

VFd_reduced: Anabaena 7120 vegetative Ferredoxin2 – 3 mM

sample_conditions: pH*: 8.5 na; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
not availableVFd_reducednot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 400 MHz

Related Database Links:

BMRB 1439 1440 1441 2463 4441 447
PDB
DBJ BAB75847
GB AAA22021
PRF 1001142A
REF WP_010998287
SP P00253 P0A3C7 P0A3C8
AlphaFold P00253 P0A3C7 P0A3C8