BMRB Entry 4438

Name: 1H, 13C and 15N chemical Shift Assignments for SCP2
Published: 2000-03-23

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4438

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical Shift Assignments for SCP2

Deposition date:

1999-10-14

Original release date:

2000-03-23

Authors:

Lopez Garcia, Francisco; Szyperski, Thomas; Dyer, James; Choinowski, Thomas; Seedorf, Udo; Hauser, Helmut; Wuthrich, Kurt

Citation:

Citation: Lopez Garcia, Francisco; Szyperski, Thomas; Dyer, James; Choinowski, Thomas; Seedorf, Udo; Hauser, Helmut; Wuthrich, Kurt. "NMR structure of the sterol carrier protein-2; implications for the biological role" J. Mol. Biol. 295, 595-603 (2000).
PubMed: 10623549

Assembly members:

Assembly members:
Sterol carrier protein 2, polymer, 123 residues, 13242.3 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T/hSCP2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sterol carrier protein 2: SSASDGFKANLVFKEIEKKL EEEGEQFVKKIGGIFAFKVK DGPGGKEATWVVDVKNGKGS VLPNSDKKADCTITMADSDF LALMTGKMNPQSAFFQGKLK ITGNMGLAMKLQNLQLQPGN AKL

Data sets:

assigned_chemical_shifts
- shiftset1

Data type	Count
1H chemical shifts	755
13C chemical shifts	301
15N chemical shifts	124

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SCP-2	1

Entities:

Entity 1, SCP-2 123 residues - 13242.3 Da.

1

SER

ALA

SER

ASP

GLY

PHE

LYS

ALA

ASN

2

LEU

VAL

PHE

LYS

GLU

ILE

GLU

LYS

LEU

3

GLU

GLY

GLU

GLN

PHE

VAL

LYS

4

ILE

GLY

ILE

PHE

ALA

PHE

LYS

VAL

LYS

5

ASP

GLY

PRO

GLY

LYS

GLU

ALA

THR

TRP

6

VAL

ASP

VAL

LYS

ASN

GLY

LYS

GLY

SER

7

VAL

LEU

PRO

ASN

SER

ASP

LYS

ALA

ASP

8

CYS

THR

ILE

THR

MET

ALA

ASP

SER

ASP

PHE

9

LEU

ALA

LEU

MET

THR

GLY

LYS

MET

ASN

PRO

10

GLN

SER

ALA

PHE

GLN

GLY

LYS

LEU

LYS

11

ILE

THR

GLY

ASN

MET

GLY

LEU

ALA

MET

LYS

12

LEU

GLN

ASN

LEU

GLN

LEU

GLN

PRO

GLY

ASN

13

ALA

LYS

LEU

Samples:

sample1: Sterol carrier protein 2, [U-15N], 1.0 mM

sample2: Sterol carrier protein 2, [U-13C; U-15N], 1 mM

conditions_set1: pH: 6.0; temperature: 309 K; ionic strength: 0.45 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	conditions_set1
1H-15N TOCSY	not available	not available	conditions_set1
HNCACBCA(CO)NH	not available	not available	conditions_set1
1H-13C-NOESY	not available	not available	conditions_set1
HCCH-COSY	not available	not available	conditions_set1
four channels, Z-gradient, triple resonance probe	not available	not available	conditions_set1

Software:

PROSA v4.3 - Processing of NMR data

XEASY - NMR spectral analysis

DYANA v1.5 - Structure calculation, Torsion angle dynamics

NMR spectrometers:

Varian Unity+ 750 MHz
Bruker AMX 600 MHz

PDB	1C44 1QND 2C0L
DBJ	BAG35708 BAG57810 BAG58208 BAG64455
EMBL	CAH91926
GB	AAA03557 AAA03558 AAA03559 AAB41286 AAH05911
REF	NP_001007100 NP_001007101 NP_001126116 NP_001180528 NP_001180529
SP	P22307
AlphaFold	P22307