BMRB Entry 4433

Title:
1H-NMR Spectral Assignment of Second Transmembrane Segments of Human Glycine Receptor
Deposition date:
1999-10-05
Original release date:
2003-04-23
Authors:
Tang, Pei; Zubrzycki, Igor; Hu, Jian; Xu, Yan
Citation:

Citation: Tang, Pei; Mandal, P.; Xu, Yan. "NMR structures of the Second Transmembrane domain of the human glycine receptor alpha(1) subunit: Model of pore architecture and channel gating."  Biophys. J. 83, 252-262 (2002).
PubMed: 12080117

Assembly members:

Assembly members:
Neurotransmitter-gated receptor, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neurotransmitter-gated receptor: PARVGLGITTVLTMTTQYSG SRA

Data typeCount
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GlyR alpha 1 M2 (S267Y)1

Entities:

Entity 1, GlyR alpha 1 M2 (S267Y) 23 residues - Formula weight is not available

1   PROALAARGVALGLYLEUGLYILETHRTHR
2   VALLEUTHRMETTHRTHRGLNTYRSERGLY
3   SERARGALA

Samples:

sample_one: Neurotransmitter-gated receptor 3.1 mM; SDS 677 mM

sample_two: Neurotransmitter-gated receptor 3.1 mM; DPC mM

sample_conditions: pH: 4.8 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-NOESYnot availablenot availablenot available
1H-TOCSYnot availablenot availablenot available

Software:

NMRPipe - Data process

SPARKY - Data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz