BMRB Entry 4430

Title:
High resolution solution structure of apo rabbit calcyclin   PubMed: 10212984
Deposition date:
1999-10-04
Original release date:
2000-10-02
Authors:
Maler, L.; Potts, B.; Chazin, W.
Citation:

Citation: Maler, L.; Potts, B.; Chazin, W.. "High resolution solution structure of apo calcyclin and structural variations in the S100 family of calcium-binding proteins"  J. Biomol. NMR 13, 233-247 (1999).

Assembly members:

Assembly members:
CALCYCLIN (RABBIT), polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: European rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET1120

Experimental source:

Natural source:   Common Name: European rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET1120

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts667
13C chemical shifts296
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calcyclin subunit A1
2calcyclin subunit B1

Entities:

Entity 1, calcyclin subunit A 90 residues - Formula weight is not available

1   METALASERPROLEUASPGLNALAILEGLY
2   LEULEUILEGLYILEPHEHISLYSTYRSER
3   GLYLYSGLUGLYASPLYSHISTHRLEUSER
4   LYSLYSGLULEULYSGLULEUILEGLNLYS
5   GLULEUTHRILEGLYSERLYSLEUGLNASP
6   ALAGLUILEVALLYSLEUMETASPASPLEU
7   ASPARGASNLYSASPGLNGLUVALASNPHE
8   GLNGLUTYRILETHRPHELEUGLYALALEU
9   ALAMETILETYRASNGLUALALEULYSGLY

Samples:

sample_1: CALCYCLIN (RABBIT), [U-13C; U-15N], 1.5 mM; Tris-d11 50 mM; NaN3 0.05%

sample_cond_1: pH: 7.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D NOESYsample_1not availablesample_cond_1
4D NOESYsample_1not availablesample_cond_1

Software:

Felix v95.0 - bookeeping of chemical shifts, manual assignment of chamical shifts, automated peak assignments

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 15418
PDB
DBJ BAA01707
REF NP_001182671
SP P30801
AlphaFold P30801

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks