BMRB Entry 4429

Name: 1H and 15N Chemical Shift Assignments for ribosomal protein L7
Published: 2002-07-12

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PDB ID: 1rqu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4429
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shift Assignments for ribosomal protein L7

Deposition date:

1999-10-03

Original release date:

2002-07-12

Authors:

Bocharov, Eduard

Citation:

Citation: Bocharov, Eduard; Gudkov, Anatolij; Arseniev, Alexandr. "Topology of the secondary structure elements of ribosomal protein L7/L12 from E.coli in solutio" FEBS Lett. 379, 291-294 (1996).

Assembly members:

Assembly members:
E.coli ribosomal protein L7/L12, polymer, 120 residues, 12164 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E.coli ribosomal protein L7/L12: SITKDQIIEAVAAMSVMDVV ELISAMEEKFGVSAAAAVAV AAGPVEAAEEKTEFDVILKA AGANKVAVIKAVRGATGLGL KEAKDLVESAPAALKEGVSK DDAEALKKALEEAGAEVEVK

Data sets:

assigned_chemical_shifts
- chem_shift_set_1
coupling_constants
- coupling_constants_set_1

Data type	Count
1H chemical shifts	823
15N chemical shifts	120
coupling constants	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	L7 dimer subunit A	1
2	L7 dimer subunit B	1

Entities:

Entity 1, L7 dimer subunit A 120 residues - 12164 Da.

1	SER	ILE	THR	LYS	ASP	GLN	ILE	ILE	GLU	ALA
2	VAL	ALA	ALA	MET	SER	VAL	MET	ASP	VAL	VAL
3	GLU	LEU	ILE	SER	ALA	MET	GLU	GLU	LYS	PHE
4	GLY	VAL	SER	ALA	ALA	ALA	ALA	VAL	ALA	VAL
5	ALA	ALA	GLY	PRO	VAL	GLU	ALA	ALA	GLU	GLU
6	LYS	THR	GLU	PHE	ASP	VAL	ILE	LEU	LYS	ALA
7	ALA	GLY	ALA	ASN	LYS	VAL	ALA	VAL	ILE	LYS
8	ALA	VAL	ARG	GLY	ALA	THR	GLY	LEU	GLY	LEU
9	LYS	GLU	ALA	LYS	ASP	LEU	VAL	GLU	SER	ALA
10	PRO	ALA	ALA	LEU	LYS	GLU	GLY	VAL	SER	LYS
11	ASP	ASP	ALA	GLU	ALA	LEU	LYS	LYS	ALA	LEU
12	GLU	GLU	ALA	GLY	ALA	GLU	VAL	GLU	VAL	LYS

Samples:

sample_1: E.coli ribosomal protein L7/L12, [U-95% 15N], 1.0 mM

sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HMQC	sample_1	not available	sample_cond_1
1H-15N HSQC	sample_1	not available	sample_cond_1
1H-15N TOCSY-HSQC	sample_1	not available	sample_cond_1
1H-15N NOESY-HSQC	sample_1	not available	sample_cond_1
1H TOCSY	sample_1	not available	sample_cond_1
1H NOESY	sample_1	not available	sample_cond_1

Software:

XEASY v1.2.11 - peak assignments

DYANA v1.5 - structure calculation

FANTOM v4 - energy minimization

MOLMOL v2.5.1 - visualization and structure analysis

NMR spectrometers:

Varian UNITY 600 MHz

PDB	1CTF 1RQS 1RQU 1RQV 2BCW 2GYA 2GYC 2XTG 2XUX 3J5O 3J5W 3J7Z 3SGF 3UOS 4KIX
DBJ	BAB38332 BAE77334 BAG79797 BAH61123 BAI28246
EMBL	CAA23624 CAA37246 CAD09488 CAP78442 CAQ34332
GB	AAC43084 AAC76960 AAF33498 AAG59182 AAL22980
PIR	AF0933
PRF	0601198A
REF	NP_312936 NP_418413 NP_457918 NP_463021 NP_709781
SP	A6TGN9 A7ZUK0 A8A785 A8AKU0 A9N0J3
AlphaFold	A6TGN9 A7ZUK0 A8A785 A8AKU0 A9N0J3 A6TGN9 A7ZUK0 A8A785 A8AKU0 A9N0J3

1	SER	ILE	THR	LYS	ASP	GLN	ILE	ILE	GLU	ALA
2	VAL	ALA	ALA	MET	SER	VAL	MET	ASP	VAL	VAL
3	GLU	LEU	ILE	SER	ALA	MET	GLU	GLU	LYS	PHE
4	GLY	VAL	SER	ALA	ALA	ALA	ALA	VAL	ALA	VAL
5	ALA	ALA	GLY	PRO	VAL	GLU	ALA	ALA	GLU	GLU
6	LYS	THR	GLU	PHE	ASP	VAL	ILE	LEU	LYS	ALA
7	ALA	GLY	ALA	ASN	LYS	VAL	ALA	VAL	ILE	LYS
8	ALA	VAL	ARG	GLY	ALA	THR	GLY	LEU	GLY	LEU
9	LYS	GLU	ALA	LYS	ASP	LEU	VAL	GLU	SER	ALA
10	PRO	ALA	ALA	LEU	LYS	GLU	GLY	VAL	SER	LYS
11	ASP	ASP	ALA	GLU	ALA	LEU	LYS	LYS	ALA	LEU
12	GLU	GLU	ALA	GLY	ALA	GLU	VAL	GLU	VAL	LYS

1	SER	ILE	THR	LYS	ASP	GLN	ILE	ILE	GLU	ALA
2	VAL	ALA	ALA	MET	SER	VAL	MET	ASP	VAL	VAL
3	GLU	LEU	ILE	SER	ALA	MET	GLU	GLU	LYS	PHE
4	GLY	VAL	SER	ALA	ALA	ALA	ALA	VAL	ALA	VAL
5	ALA	ALA	GLY	PRO	VAL	GLU	ALA	ALA	GLU	GLU
6	LYS	THR	GLU	PHE	ASP	VAL	ILE	LEU	LYS	ALA
7	ALA	GLY	ALA	ASN	LYS	VAL	ALA	VAL	ILE	LYS
8	ALA	VAL	ARG	GLY	ALA	THR	GLY	LEU	GLY	LEU
9	LYS	GLU	ALA	LYS	ASP	LEU	VAL	GLU	SER	ALA
10	PRO	ALA	ALA	LEU	LYS	GLU	GLY	VAL	SER	LYS
11	ASP	ASP	ALA	GLU	ALA	LEU	LYS	LYS	ALA	LEU
12	GLU	GLU	ALA	GLY	ALA	GLU	VAL	GLU	VAL	LYS