BMRB Entry 4423
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4423
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Citation: Kowalski, K.; Czolij, R.; King, G.; Crossley, M.; Mackay, J.. "The Solution Structure of the N-terminal Zinc Finger of GATA-1 Reveals a
Specific Binding Face for the Transcriptional Co-factor FO" J. Biomol. NMR 13, 249-262 (1999).
PubMed: 10212985
Assembly members:
GATA-1, polymer, 46 residues, 5189 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
GATA-1: GSEARECVNCGATATPLWRR
DRTGHYLCNACGLYHKMNGQ
NRPLIR
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 303 |
| 15N chemical shifts | 36 |
| coupling constants | 20 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GATA-1 | 1 |
| 2 | ZN | 2 |
Entities:
Entity 1, GATA-1 46 residues - 5189 Da.
| 1 | GLY | SER | GLU | ALA | ARG | GLU | CYS | VAL | ASN | CYS | ||||
| 2 | GLY | ALA | THR | ALA | THR | PRO | LEU | TRP | ARG | ARG | ||||
| 3 | ASP | ARG | THR | GLY | HIS | TYR | LEU | CYS | ASN | ALA | ||||
| 4 | CYS | GLY | LEU | TYR | HIS | LYS | MET | ASN | GLY | GLN | ||||
| 5 | ASN | ARG | PRO | LEU | ILE | ARG |
Entity 2, ZN - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: GATA-1 4 mM; Tris 10 mM; TCEP 5 mM; ZnSO4 5 mM
sample_2: GATA-1, [U-15N], 2 mM; Tris 20 mM; TCEP 3 mM; ZnSO4 3 mM
sample_cond_1: pH: 5.4; temperature: 288 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | not available | not available | sample_cond_1 |
| DQFCOSY | not available | not available | sample_cond_1 |
| NOESY | not available | not available | sample_cond_1 |
| ECOSY | not available | not available | sample_cond_1 |
| HSQC | not available | not available | sample_cond_1 |
| TOCSY-HSQC | not available | not available | sample_cond_1 |
| NOESY-HSQC | not available | not available | sample_cond_1 |
| J-MODULATED HMQC | not available | not available | sample_cond_1 |
Software:
XWINNMR - 1D/2D data processing
Aurelia - 3D data processing
DIANA - distance geometry
X-Plor - dynamical simulated-annealing
MOLMOL - molecular visualisation
NMR spectrometers:
- Bruker AMX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAG68610 BAK39708 |
| GB | AFX60070 EAW50750 |
| REF | XP_007507788 XP_008156485 XP_008695477 XP_009437329 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks