BMRB Entry 4411
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4411
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NMR-STAR v3 text file.
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Citation: Bocharov, Eduard; Blommers, Marcel; Kuhla, J.; Arvinte, T.; Burgi, R.; Arseniev, Alexandr. "Letter to the Editor: Sequence-specific 1H and 15N assignment and secondary
structure of transforming growth factor beta3" J. Biomol. NMR 16, 179-180 (2000).
Assembly members:
human transforming growth factor beta3, polymer, 112 residues, 12706 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human transforming growth factor beta3: ALDTNYCFRNLEENCCVRPL
YIDFRQDLGWKWVHEPKGYY
ANFCSGPCPYLRSADTTHST
VLGLYNTLNPEASASPCCVP
QDLEPLTILYYVGRTPKVEQ
LSNMVVKSCKCS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 196 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TGF-beta3 subunit A | 1 |
| 2 | TGF-beta3 subunit B | 1 |
Entities:
Entity 1, TGF-beta3 subunit A 112 residues - 12706 Da.
| 1 | ALA | LEU | ASP | THR | ASN | TYR | CYS | PHE | ARG | ASN | ||||
| 2 | LEU | GLU | GLU | ASN | CYS | CYS | VAL | ARG | PRO | LEU | ||||
| 3 | TYR | ILE | ASP | PHE | ARG | GLN | ASP | LEU | GLY | TRP | ||||
| 4 | LYS | TRP | VAL | HIS | GLU | PRO | LYS | GLY | TYR | TYR | ||||
| 5 | ALA | ASN | PHE | CYS | SER | GLY | PRO | CYS | PRO | TYR | ||||
| 6 | LEU | ARG | SER | ALA | ASP | THR | THR | HIS | SER | THR | ||||
| 7 | VAL | LEU | GLY | LEU | TYR | ASN | THR | LEU | ASN | PRO | ||||
| 8 | GLU | ALA | SER | ALA | SER | PRO | CYS | CYS | VAL | PRO | ||||
| 9 | GLN | ASP | LEU | GLU | PRO | LEU | THR | ILE | LEU | TYR | ||||
| 10 | TYR | VAL | GLY | ARG | THR | PRO | LYS | VAL | GLU | GLN | ||||
| 11 | LEU | SER | ASN | MET | VAL | VAL | LYS | SER | CYS | LYS | ||||
| 12 | CYS | SER |
Samples:
sample_1: human transforming growth factor beta3, [U-95% 15N], 1.0 mM; dioxane-d8 8%; methanol-d3 3%
sample_cond_1: pH: 2.90; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | sample_cond_1 |
| 1H-15N TOCSY-HSQC | sample_1 | not available | sample_cond_1 |
| 1H-15N NOESY-HSQC | sample_1 | not available | sample_cond_1 |
| 1H TOCSY | sample_1 | not available | sample_cond_1 |
| 1H NOESY | sample_1 | not available | sample_cond_1 |
Software:
XEASY v1.2.11 - peak assignments
NMR spectrometers:
- Varian UNITY 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE21215 BAE26357 BAE28523 BAE36326 BAE36968 |
| EMBL | CAA32362 CAA32363 CAA33024 CAR70088 |
| GB | AAA40422 AAA49089 AAA61161 AAA67915 AAC79727 |
| REF | NP_001094653 NP_001244404 NP_003230 NP_033394 NP_037306 |
| SP | P10600 P15203 P17125 Q07258 |
| TPG | DAA25016 |
| AlphaFold | P10600 P15203 P17125 Q07258 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks