BMRB Entry 4411

Title:
Backbone 1H and 15N Chemical Shift Assignments for Transforming Growth Factor beta
Deposition date:
1999-09-16
Original release date:
2000-03-17
Authors:
Bocharov, Eduard; Blommers, Marcel; Kuhla, J.; Arvinte, T.; Burgi, R.; Arseniev, Alexandr
Citation:

Citation: Bocharov, Eduard; Blommers, Marcel; Kuhla, J.; Arvinte, T.; Burgi, R.; Arseniev, Alexandr. "Letter to the Editor: Sequence-specific 1H and 15N assignment and secondary structure of transforming growth factor beta3"  J. Biomol. NMR 16, 179-180 (2000).

Assembly members:

Assembly members:
human transforming growth factor beta3, polymer, 112 residues, 12706 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo Sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts90
1H chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TGF-beta3 subunit A1
2TGF-beta3 subunit B1

Entities:

Entity 1, TGF-beta3 subunit A 112 residues - 12706 Da.

1   ALALEUASPTHRASNTYRCYSPHEARGASN
2   LEUGLUGLUASNCYSCYSVALARGPROLEU
3   TYRILEASPPHEARGGLNASPLEUGLYTRP
4   LYSTRPVALHISGLUPROLYSGLYTYRTYR
5   ALAASNPHECYSSERGLYPROCYSPROTYR
6   LEUARGSERALAASPTHRTHRHISSERTHR
7   VALLEUGLYLEUTYRASNTHRLEUASNPRO
8   GLUALASERALASERPROCYSCYSVALPRO
9   GLNASPLEUGLUPROLEUTHRILELEUTYR
10   TYRVALGLYARGTHRPROLYSVALGLUGLN
11   LEUSERASNMETVALVALLYSSERCYSLYS
12   CYSSER

Samples:

sample_1: human transforming growth factor beta3, [U-95% 15N], 1.0 mM; dioxane-d8 8%; methanol-d3 3%

sample_cond_1: pH: 2.90; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablesample_cond_1
1H-15N TOCSY-HSQCsample_1not availablesample_cond_1
1H-15N NOESY-HSQCsample_1not availablesample_cond_1
1H TOCSYsample_1not availablesample_cond_1
1H NOESYsample_1not availablesample_cond_1

Software:

XEASY v1.2.11 - peak assignments

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB
DBJ BAE21215 BAE26357 BAE28523 BAE36326 BAE36968
EMBL CAA32362 CAA32363 CAA33024 CAR70088
GB AAA40422 AAA49089 AAA61161 AAA67915 AAC79727
REF NP_001094653 NP_001244404 NP_003230 NP_033394 NP_037306
SP P10600 P15203 P17125 Q07258
TPG DAA25016
AlphaFold P10600 P15203 P17125 Q07258

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks