BMRB Entry 4394

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4394
MolProbity Validation Chart

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Title:
Translation initiation factor IF3 from Escherichia coli Ribosome binding domain (residues 84-180)
Deposition date:
1999-09-09
Original release date:
1999-11-29
Authors:
DE COCK, E.; GARCIA, C.; DARDEL, F.
Citation:

Citation: DE COCK, E.; BLANQUET, S.; LALLEMAND, J.; DARDEL, F.. "INTERACTION OF E. COLI TRANSLATION INITIATION FACTOR IF3 WITH THE RIBOSOME"  .

Assembly members:

Assembly members:
TRANSLATION INITIATION FACTOR IF3, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: ESCHERICHIA COLI

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: pTRC99a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TRANSLATION INITIATION FACTOR IF3: MEFQKKKQKVIQVKEIKFRP GTDEGDYQVKLRSLIRFLEE GDKAKITLRFRGREMAHQQI GMEVLNRVKDDLQELAVVES FPTKIEGRQMIMVLAPKKKQ

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts92
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IF3 ribosome binding domain1

Entities:

Entity 1, IF3 ribosome binding domain 100 residues - Formula weight is not available

1   METGLUPHEGLNLYSLYSLYSGLNLYSVAL
2   ILEGLNVALLYSGLUILELYSPHEARGPRO
3   GLYTHRASPGLUGLYASPTYRGLNVALLYS
4   LEUARGSERLEUILEARGPHELEUGLUGLU
5   GLYASPLYSALALYSILETHRLEUARGPHE
6   ARGGLYARGGLUMETALAHISGLNGLNILE
7   GLYMETGLUVALLEUASNARGVALLYSASP
8   ASPLEUGLNGLULEUALAVALVALGLUSER
9   PHEPROTHRLYSILEGLUGLYARGGLNMET
10   ILEMETVALLEUALAPROLYSLYSLYSGLN

Samples:

sample_1: TRANSLATION INITIATION FACTOR IF3, [U-98% 13C; U-95% 15N], 1.5 mM; potassium phosphate 20 mM

sample_2: TRANSLATION INITIATION FACTOR IF3, [U-98% 15N], 1.5 mM; potassium phosphate 20 mM

sample_cond_1: ionic strength: 20 mM; pH*: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablesample_cond_1
3D HNCAnot availablenot availablesample_cond_1
3D HNHBnot availablenot availablesample_cond_1
3D 1H-1H-15N NOESYnot availablenot availablesample_cond_1
3D 13C-1H-1H NOESYnot availablenot availablesample_cond_1

Software:

GIFA v4.0 - NMR data processing and graphic display

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAA15485 BAB35848 BAG66478 BAG77410 BAH63902
EMBL CAA23561 CAD02019 CAP76214 CAQ32193 CAR33292
GB AAA51467 AAC36811 AAC36814 AAC74788 AAG56705
PIR AB0706 E85780
REF NP_310452 NP_416233 NP_456178 NP_460300 NP_707396
SP B1IPL0 B7USA1 C4ZYI0 P0A707 P0A708
AlphaFold B1IPL0 B7USA1 C4ZYI0 P0A707 P0A708

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