BMRB Entry 4388

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4388

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Title:
Backbone NMR Assignments of a High Molecular Weight Protein (47kD), Cyclic AMP Receptor Protein (apo-CRP)
Deposition date:
1999-09-04
Original release date:
2000-03-20
Authors:
Won, Hyung-Sik; Yamazaki, T.; Lee, Tae-Woo; Jee, Jun-Goo; Yoon, Mi-Kyung; Park, Sang-Ho; Otomo, T.; Aiba, H.; Kyogoku, Y.; Lee, Bong-Jin
Citation:

Citation: Won, Hyung-Sik; Yamazaki, T.; Lee, Tae-Woo; Jee, Jun-Goo; Yoon, Mi-Kyung; Park, Sang-Ho; Otomo, T.; Aiba, H.; Kyogoku, Y.; Lee, Bong-Jin. "Letter to the Editor: Backbone NMR assignments of a high molecular weight protein (47 kDa), cyclic AMP receptor protein (apo-CRP)"  J. Biomol. NMR 16, 79-80 (2000).

Assembly members:

Assembly members:
Cyclic AMP Receptor Protein, polymer, 209 residues, 47000 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLCRP1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cyclic AMP Receptor Protein: VLGKPQTDPTLEWFLSHCHI HKYPSKSTLIHQGEKAETLY YIVKGSVAVLIKDEEGKEMI LSYLNQGDFIGELGLFEEGQ ERSAWVRAKTACEVAEISYK KFRQLIQVNPDILMRLSAQM ARRLQVTSEKVGNLAFLDVT GRIAQTLLNLAKQPDAMTHP DGMQIKITRQEIGQIVGCSR ETVGRILKMLEDQNLISAHG KTIVVYGTR

Data sets:
Data typeCount
13C chemical shifts579
15N chemical shifts191
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRP subunit 11
2CRP subunit 21

Entities:

Entity 1, CRP subunit 1 209 residues - 47000 Da.

1   VALLEUGLYLYSPROGLNTHRASPPROTHR
2   LEUGLUTRPPHELEUSERHISCYSHISILE
3   HISLYSTYRPROSERLYSSERTHRLEUILE
4   HISGLNGLYGLULYSALAGLUTHRLEUTYR
5   TYRILEVALLYSGLYSERVALALAVALLEU
6   ILELYSASPGLUGLUGLYLYSGLUMETILE
7   LEUSERTYRLEUASNGLNGLYASPPHEILE
8   GLYGLULEUGLYLEUPHEGLUGLUGLYGLN
9   GLUARGSERALATRPVALARGALALYSTHR
10   ALACYSGLUVALALAGLUILESERTYRLYS
11   LYSPHEARGGLNLEUILEGLNVALASNPRO
12   ASPILELEUMETARGLEUSERALAGLNMET
13   ALAARGARGLEUGLNVALTHRSERGLULYS
14   VALGLYASNLEUALAPHELEUASPVALTHR
15   GLYARGILEALAGLNTHRLEULEUASNLEU
16   ALALYSGLNPROASPALAMETTHRHISPRO
17   ASPGLYMETGLNILELYSILETHRARGGLN
18   GLUILEGLYGLNILEVALGLYCYSSERARG
19   GLUTHRVALGLYARGILELEULYSMETLEU
20   GLUASPGLNASNLEUILESERALAHISGLY
21   LYSTHRILEVALVALTYRGLYTHRARG

Samples:

sample_1: Cyclic AMP Receptor Protein, [U-90% 2H; U-95% 13C; U-95% 15N], 0.6 mM

Ex-cond_1: pH*: 6.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableEx-cond_1
1H-15N NOESY-HSQCsample_1not availableEx-cond_1
CT-HNCAsample_1not availableEx-cond_1
CT-HN(CO)CAsample_1not availableEx-cond_1
CT-HN(CA)CBsample_1not availableEx-cond_1
CT-HN(COCA)CBsample_1not availableEx-cond_1
CT-HNCOsample_1not availableEx-cond_1

Software:

NMRPipe -

NMRDraw -

NMRView -

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz
  • Bruker AMX 500 MHz

Related Database Links:

BMRB 19144 19145
PDB
DBJ BAB37631 BAC10627 BAE75576 BAE77933 BAG79143
EMBL CAA04867 CAA61609 CAC07215 CAD08147 CAE12690
GB AAA23601 AAA25058 AAA26515 AAA27039 AAA58154
PIR A44903 AG1002
REF NP_312235 NP_417816 NP_458435 NP_462369 NP_709132
SP P0A2T6 P0A2T7 P0ACJ8 P0ACJ9 P0ACK0
AlphaFold P0A2T6 P0A2T7 P0ACJ8 P0ACJ9 P0ACK0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks