BMRB Entry 4385
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4385
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Citation: Kozlov, Guennadi; Ekiel, Irena; Beglova, Natalia; Yee, Adelinda; Dharamsi, Akil; Engel, Asaph; Siddiqui, Nadeem; Nong, Andrew; Gehring, Kalle. "Rapid Fold and Sructure Determination of the Archaeal Translation Elongation
Factor 1beta from Methanobacterium thermoautotrophicum" J. Biomol. NMR 17, 187-194 (2000).
Assembly members:
elongation factor-1beta, polymer, 109 residues, 9550 Da.
Natural source: Common Name: Methanobacterium thermoautotrophicum Taxonomy ID: 2166 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanobacterium thermoautotrophicum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
elongation factor-1beta: MGSSHHHHHHSSGLVPRGSH
MGDVVATIKVMPESPDVDLE
ALKKEIQERIPEGTELHKID
EEPIAFGLVALNVMVVVGDA
EGGTEAAEESLSGIEGVSNI
EVTDVRRLM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 340 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 585 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | elongation factor-1beta | 1 |
Entities:
Entity 1, elongation factor-1beta 109 residues - 9550 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | GLY | ASP | VAL | VAL | ALA | THR | ILE | LYS | VAL | ||||
| 4 | MET | PRO | GLU | SER | PRO | ASP | VAL | ASP | LEU | GLU | ||||
| 5 | ALA | LEU | LYS | LYS | GLU | ILE | GLN | GLU | ARG | ILE | ||||
| 6 | PRO | GLU | GLY | THR | GLU | LEU | HIS | LYS | ILE | ASP | ||||
| 7 | GLU | GLU | PRO | ILE | ALA | PHE | GLY | LEU | VAL | ALA | ||||
| 8 | LEU | ASN | VAL | MET | VAL | VAL | VAL | GLY | ASP | ALA | ||||
| 9 | GLU | GLY | GLY | THR | GLU | ALA | ALA | GLU | GLU | SER | ||||
| 10 | LEU | SER | GLY | ILE | GLU | GLY | VAL | SER | ASN | ILE | ||||
| 11 | GLU | VAL | THR | ASP | VAL | ARG | ARG | LEU | MET |
Samples:
sample_1: elongation factor-1beta, [U-98% 13C; U-98% 15N], 1.0 – 3.0 mM; sodium phosphate 50 mM; sodium chloride 150 mM; EDTA 10 mM; sodium azide 0.02%
standard_conditions: ionic strength: 0.2 M; pH: 6.3; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C-HSQC | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| 1H-13C TOCSY & COSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCACONH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian Unity 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks