BMRB Entry 4382

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4382

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Title:
1H, 13C and 15N Assigned Chemical Shifts for a Eukaryotic Rubredoxin from Guillardia theta
Deposition date:
1999-08-25
Original release date:
2000-11-30
Authors:
Schweimer, Kristian; Hoffmann, Silke; Wastl, J.; Maier, U.-G.; Roesch, Paul; Sticht, Heinrich
Citation:

Citation: Schweimer, Kristian; Hoffmann, Silke; Wastl, J.; Maier, U.-G.; Roesch, Paul; Sticht, Heinrich. "Solution Structure of a Zinc Substituted Eukaryotic Rubredoxin from the Cryptomonad Algal Guillardia theta"  Protein Sci. 9, 1474-1486 (2000).

Assembly members:

Assembly members:
Guillardia Theta Rubredoxin, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Guillardia theta   Taxonomy ID: 55529   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Guillardia theta

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Guillardia Theta Rubredoxin: MEIDEGKYECEACGYIYEPE KGDKFAGIPPGTPFVDLSDS FMCPACRSPKNQFKSIKKVI AGFAENQKYG

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts68
1H chemical shifts435

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin1
2zinc2

Entities:

Entity 1, rubredoxin 70 residues - Formula weight is not available

1   METGLUILEASPGLUGLYLYSTYRGLUCYS
2   GLUALACYSGLYTYRILETYRGLUPROGLU
3   LYSGLYASPLYSPHEALAGLYILEPROPRO
4   GLYTHRPROPHEVALASPLEUSERASPSER
5   PHEMETCYSPROALACYSARGSERPROLYS
6   ASNGLNPHELYSSERILELYSLYSVALILE
7   ALAGLYPHEALAGLUASNGLNLYSTYRGLY

Entity 2, zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Guillardia Theta Rubredoxin, [U-95% 13C; U-95% 15N], 2.6 mM

Ex-cond_1: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYsample_1not availableEx-cond_1
1H-1H TOCSYsample_1not availableEx-cond_1
1H-15N HSQCsample_1not availableEx-cond_1
1H-13C CT-HSQCsample_1not availableEx-cond_1
15N-edited NOESY(3D)sample_1not availableEx-cond_1
13C-edited NOESY(3D)sample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
HBHA(CO)NHsample_1not availableEx-cond_1
HNHAsample_1not availableEx-cond_1
HCCH-COSYsample_1not availableEx-cond_1

Software:

NMRview v4.0.1 - peak picking and visualization of the NMR data

Home written Software - In-house developed software for data processing and automatic backbone assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAB40406
REF XP_001713191

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks