BMRB Entry 4381

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4381

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Letter to the Editor:Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)

Deposition date:

1999-08-19

Original release date:

2000-03-20

Authors:

Enmon, Jennifer; de Beer, Tonny; Overduin, Michael

Citation:

Citation: Enmon, Jennifer; de Beer, Tonny; Overduin, Michael. "Letter to the Editor: Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)" J. Biomol. NMR 16, 81-82 (2000).

Assembly members:

Assembly members:
third EH domain of Eps15, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSETA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
third EH domain of Eps15: KRKTWVVSPAEKAKYDEIFL KTDKDMDGFVSGLEVREIFL KTGLPSTLLAHIWSLCDTKD CGKLSKDQFALAFHLISQKL IKGIDPPHVLTPEMIPPSDR ASLQ

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	445
15N chemical shifts	92
1H chemical shifts	713

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EH3	1

Entities:

Entity 1, EH3 104 residues - Formula weight is not available

1

LYS

ARG

LYS

THR

TRP

VAL

SER

PRO

ALA

2

GLU

LYS

ALA

LYS

TYR

ASP

GLU

ILE

PHE

LEU

3

LYS

THR

ASP

LYS

ASP

MET

ASP

GLY

PHE

VAL

4

SER

GLY

LEU

GLU

VAL

ARG

GLU

ILE

PHE

LEU

5

LYS

THR

GLY

LEU

PRO

SER

THR

LEU

ALA

6

HIS

ILE

TRP

SER

LEU

CYS

ASP

THR

LYS

ASP

7

CYS

GLY

LYS

LEU

SER

LYS

ASP

GLN

PHE

ALA

8

LEU

ALA

PHE

HIS

LEU

ILE

SER

GLN

LYS

LEU

9

ILE

LYS

GLY

ILE

ASP

PRO

HIS

VAL

LEU

10

THR

PRO

GLU

MET

ILE

PRO

SER

ASP

ARG

11

ALA

SER

LEU

GLN

Samples:

sample_1: third EH domain of Eps15, [U-13C; U-15N], 1.0 mM; d-tris 10 mM; KCl 100 mM; NaN3 2 mM; APMSF 0.010 mM; d-DTT0.1 – 40 mM; D2O 10%; H2O 90%

sample_2: third EH domain of Eps15, [U-13C; U-15N], 1.0 mM; d-tris 10 mM; KCl 100 mM; NaN3 2 mM; APMSF 0.010 mM; d-DTT0.1 – 40 mM; D2O 99.9%

condition_1: pH: 7.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCA(CO)NH	not available	not available	condition_1
HNCACB	not available	not available	condition_1
HCC-TOCSY	not available	not available	condition_1
CCC-TOCSY	not available	not available	condition_1
HCCH_TOCSY	not available	not available	condition_1
3D-15N-NOESY	not available	not available	condition_1
HNCO	not available	not available	condition_1
2D CB corelation with HD and HE	not available	not available	condition_1

Software:

NMRPipe - processing

PIPP - analysis

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz

PDB	1C07
REF	XP_006710509 XP_007976962 XP_009455980 XP_011539293 XP_011781647