BMRB Entry 4374

Name: Mutational and structural analyses of the ribonucleotide reductase inhibitor sml1 define its rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality
Published: 2002-04-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4374

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mutational and structural analyses of the ribonucleotide reductase inhibitor sml1 define its rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality

Deposition date:

1999-08-05

Original release date:

2002-04-08

Authors:

Zao, X.; Goergieva, B.; Chabes, Andrej; Domkin, Vladimir; Ippel, Hans; Schleucher, Juergen; Wijmenga, Sybren; Thelander, Lars; Rothstein, R.

Citation:

Citation: Zao, X.; Goergieva, B.; Chabes, Andrej; Domkin, Vladimir; Ippel, Hans; Schleucher, Juergen; Wijmenga, Sybren; Thelander, Lars; Rothstein, R.. "Mutational and structural analyses of the ribonucleotide reductase inhibitor sml1 define its rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality" Mol. Cell. Biol. 20, 9076-9083 (2000).

Assembly members:

Assembly members:
Sml1, polymer, 104 residues, 11834.19 Da.

Natural source:

Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: not available Genus/species: not available Saccharomyces

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sml1: MQNSQDYFYAQNRCQQQQAP STLRTVTMAEFRRVPLPPMA EVPMLSTQNSMGSSASASAS SLEMWEKDLEERLNSIDHDM NNNKFGSGELKSMFNQGKVE EMDF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts
coupling_constants
- coupling_constants_label

Data type	Count
13C chemical shifts	389
15N chemical shifts	96
1H chemical shifts	641
coupling constants	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sml1 protein	1

Entities:

Entity 1, Sml1 protein 104 residues - 11834.19 Da.

1

MET

GLN

ASN

SER

GLN

ASP

TYR

PHE

TYR

ALA

2

GLN

ASN

ARG

CYS

GLN

ALA

PRO

3

SER

THR

LEU

ARG

THR

VAL

THR

MET

ALA

GLU

4

PHE

ARG

VAL

PRO

LEU

PRO

MET

ALA

5

GLU

VAL

PRO

MET

LEU

SER

THR

GLN

ASN

SER

6

MET

GLY

SER

ALA

SER

ALA

SER

ALA

SER

7

SER

LEU

GLU

MET

TRP

GLU

LYS

ASP

LEU

GLU

8

GLU

ARG

LEU

ASN

SER

ILE

ASP

HIS

ASP

MET

9

ASN

LYS

PHE

GLY

SER

GLY

GLU

LEU

10

LYS

SER

MET

PHE

ASN

GLN

GLY

LYS

VAL

GLU

11

GLU

MET

ASP

PHE

Samples:

sample_one: Sml1 0.6 mM; H2O 90%; D2O 10%

sample_2: Sml1, [U-15N], 0.4 mM; H2O 90%; D2O 10%; sodium-phosphate buffer 25 mM; NaCl 25 mM; Dithio-threitol 10 mM

sample_3: Sml1, [U-13C; U-15N], 0.6 mM; H2O 90%; D2O 10%

conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 bar; temperature: 275 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

DBJ	GAA25391
EMBL	CAA86717 CAY81764
GB	AAS56287 AHY76409 AJP40654 AJS61834 AJS62260
REF	NP_013653
SP	Q04964
TPG	DAA09840
AlphaFold	Q04964