BMRB Entry 4370

Name: 1H and 15N chemical shift assignments for HP-RNase
Published: 2000-12-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4370

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N chemical shift assignments for HP-RNase

Deposition date:

1999-07-19

Original release date:

2000-12-14

Authors:

El-Joubary, Abderraman; Bruix, Marta; Santoro, Jorge; Cafaro, Valeria; Scognamiglio, Roberta; Di Donato, Alberto; D'Alessio, Giuseppe; Kover, Katalin; Batta, Gyula; Szilagyi, Laszlo; Rico, Manuel

Citation:

Citation: El-Joubary, Abderraman; Bruix, Marta; Santoro, Jorge; Cafaro, Valeria; Scognamiglio, Roberta; Di Donato, Alberto; D'Alessio, Giuseppe; Kover, Katalin; Batta, Gyula; Szilagyi, Laszlo; Rico, Manuel. "1H and 15N Sequential Assignment and Solution Secondary Structure of 15N Labelled Human Pancreatic Ribonuclease" J. Biomol. NMR 15, 265-266 (1999).

Assembly members:

Assembly members:
Human Pancreatic Ribonuclease, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human Pancreatic Ribonuclease: MKESRAKKFQRQHMDSDSSP SSSSTYCNQMMRRRNMTQGR CKPVNTFVHEPLVDVQNVCF QEKVTCKNGQGNCYKSNSSM HITDCRLTNGSRYPNCAYRT SPKERHIIVACEGSPYVPVH FDASVEDS

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
15N chemical shifts	143
1H chemical shifts	795

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP-RNase	1

Entities:

Entity 1, HP-RNase 128 residues - Formula weight is not available

1

MET

LYS

GLU

SER

ARG

ALA

LYS

PHE

GLN

2

ARG

GLN

HIS

MET

ASP

SER

ASP

SER

PRO

3

SER

THR

TYR

CYS

ASN

GLN

MET

4

MET

ARG

ASN

MET

THR

GLN

GLY

ARG

5

CYS

LYS

PRO

VAL

ASN

THR

PHE

VAL

HIS

GLU

6

PRO

LEU

VAL

ASP

VAL

GLN

ASN

VAL

CYS

PHE

7

GLN

GLU

LYS

VAL

THR

CYS

LYS

ASN

GLY

GLN

8

GLY

ASN

CYS

TYR

LYS

SER

ASN

SER

MET

9

HIS

ILE

THR

ASP

CYS

ARG

LEU

THR

ASN

GLY

10

SER

ARG

TYR

PRO

ASN

CYS

ALA

TYR

ARG

THR

11

SER

PRO

LYS

GLU

ARG

HIS

ILE

VAL

ALA

12

CYS

GLU

GLY

SER

PRO

TYR

VAL

PRO

VAL

HIS

13

PHE

ASP

ALA

SER

VAL

GLU

ASP

SER

Samples:

sample_1: Human Pancreatic Ribonuclease1 – 1.5 mM

sample_2: Human Pancreatic Ribonuclease, [U-95% 15N], 1 – 1.5 mM

sample_conditions_1: ionic strength: 0.6 .; pH: 4.2; temperature: 293 K

sample_conditions_2: ionic strength: 0.6 M; pH: 4.2; temperature: 298 K

sample_conditions_3: ionic strength: 0.6 M; pH: 4.2; temperature: 303 K

sample_conditions_4: ionic strength: 0.6 M; pH: 4.2; temperature: 308 K

sample_conditions_5: ionic strength: 0.6 M; pH*: 4.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
COSY	not available	not available	not available
TOCSY	not available	not available	not available
NOESY	not available	not available	not available
15N-HSQC	not available	not available	not available
15N-HSQC-TOCSY	not available	not available	not available
15N-HSQC-NOESY	not available	not available	not available

Software:

XEASY v1.3.13 - semi-automated peak assignments

NMR spectrometers:

Bruker AMX 600 MHz

PDB	1E21 1Z7X 2E0J 2E0L 2K11 2Q4G
DBJ	BAA05124 BAG35036
EMBL	CAA44718 CAA55817 CAG29314 CAI47727
GB	AAB35096 AAH05324 AAH22882 AAL87049 AAL87050
REF	NP_001009108 NP_002924 NP_937875 NP_937877 NP_937878
SP	P07998 Q8SQ11 Q8SQ13 Q8SQ14
TPE	CDG31913 CDG31928 CDG31954
AlphaFold	P07998 Q8SQ11 Q8SQ13 Q8SQ14