BMRB Entry 4369

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4369

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Title:
1H, 15N and 13C Assigned Chemical Shifts of S19 from Thermus thermophilus
Deposition date:
1999-07-19
Original release date:
1999-10-26
Authors:
Helgstrand, Magnus; Rak, Alexey; Allard, Peter; Davydova, Natalia; Garber, Maria; Hard, Torleif
Citation:

Citation: Helgstrand, Magnus; Rak, Alexey; Allard, Peter; Davydova, Natalia; Garber, Maria; Hard, Torleif. "1H, 15N and 13C Assigned Chemical Shifts of S19 from Thermus thermophilus"  (1999).

Assembly members:

Assembly members:
S19, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pACA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S19: MPRSLKKGVFVDDHLLEKVL ELNAKGEKRLIKTWSRRSTI VPEMVGHTIAVYNGKQHVPV YITENMVGHKLGEFAPTRTY RGHGKEAKATKKK

Data sets:
Data typeCount
13C chemical shifts172
15N chemical shifts72
1H chemical shifts451

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S191

Entities:

Entity 1, S19 93 residues - Formula weight is not available

1   METPROARGSERLEULYSLYSGLYVALPHE
2   VALASPASPHISLEULEUGLULYSVALLEU
3   GLULEUASNALALYSGLYGLULYSARGLEU
4   ILELYSTHRTRPSERARGARGSERTHRILE
5   VALPROGLUMETVALGLYHISTHRILEALA
6   VALTYRASNGLYLYSGLNHISVALPROVAL
7   TYRILETHRGLUASNMETVALGLYHISLYS
8   LEUGLYGLUPHEALAPROTHRARGTHRTYR
9   ARGGLYHISGLYLYSGLUALALYSALATHR
10   LYSLYSLYS

Samples:

sample_1: S19, [U-13C; U-15N], 0.9 – 1.5 mM

Ex-cond_1: ionic strength: 0.250 M; pH: 6.75; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
CBCANHsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
C(CO)NH-TOCSYsample_1not availableEx-cond_1
HCCH-TOCSYsample_1not availableEx-cond_1
(HB)CB(CGCD)HDsample_1not availableEx-cond_1
(HB)CB(CGCDCE)HEsample_1not availableEx-cond_1
15N-edited NOESY-HSQCsample_1not availableEx-cond_1
15N-edited TOCSY-HSQCsample_1not availableEx-cond_1
3D HNHAsample_1not availableEx-cond_1
2D NOESYsample_1not availableEx-cond_1
2D TOCSYsample_1not availableEx-cond_1

Software:

VNMR v5.3 - Data processing

Pronto v970523 - Assignment

NMR spectrometers:

  • Varian Unity-Inova 500 MHz
  • Varian Unity-Inova 600 MHz
  • Varian Unity-Inova 800 MHz

Related Database Links:

PDB
DBJ BAD71511
EMBL CAA58878
GB AAS81666 ADW22843 AEG34101 AEV15515 AFH38266
REF WP_003043865 WP_011173711 WP_018111295 WP_028493563 WP_038048058
SP P62660 P80381 Q5SHP2
AlphaFold P62660 P80381 Q5SHP2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks