BMRB Entry 4363
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4363
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Citation: Riek, Roland; Precheur, Benedicte; Wang, Yunyuan; Mackay, Elaine; Wider, Gerhard; Guntert, Peter; Liu, Aizhuo; Kaegi, Jeremias; Wuthrich, Kurt. "NMR Structure of the Sea Urchin (Strongylocentrotus purpuratus) Metallothionein
MTA" J. Mol. Biol. 291, 417-428 (1999).
Assembly members:
Metallothionein MTA, polymer, 63 residues, Formula weight is not available
CD, non-polymer, 112.411 Da.
Natural source: Common Name: purple urchin Taxonomy ID: 7668 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Strongylocentrotus purpuratus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Metallothionein MTA: PDVKCVCCTEGKECACFGQD
CCVTGECCKDGTCCGICTNA
ACKCANGCKCGSGCSCTEGN
CAC
- assigned_chemical_shifts
| Data type | Count |
| 113Cd chemical shifts | 7 |
| 1H chemical shifts | 321 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Metallothionein MTA | 1 |
| 2 | CdII-1 | 2 |
| 3 | CdII-2 | 2 |
| 4 | CdII-3 | 2 |
| 5 | CdII-4 | 2 |
| 6 | CdII-5 | 2 |
| 7 | CdII-6 | 2 |
| 8 | CdII-7 | 2 |
Entities:
Entity 1, Metallothionein MTA 63 residues - Formula weight is not available
| 1 | PRO | ASP | VAL | LYS | CYS | VAL | CYS | CYS | THR | GLU | ||||
| 2 | GLY | LYS | GLU | CYS | ALA | CYS | PHE | GLY | GLN | ASP | ||||
| 3 | CYS | CYS | VAL | THR | GLY | GLU | CYS | CYS | LYS | ASP | ||||
| 4 | GLY | THR | CYS | CYS | GLY | ILE | CYS | THR | ASN | ALA | ||||
| 5 | ALA | CYS | LYS | CYS | ALA | ASN | GLY | CYS | LYS | CYS | ||||
| 6 | GLY | SER | GLY | CYS | SER | CYS | THR | GLU | GLY | ASN | ||||
| 7 | CYS | ALA | CYS |
Entity 2, CdII-1 - Cd - 112.411 Da.
| 1 | CD |
Samples:
sample_1: Metallothionein MTA, [U-113Cd], 4 mM
cond_1: pH: 7.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | cond_1 |
Software:
DYANA v1.1 -
NMR spectrometers:
- Varian Unity+ 750 MHz