BMRB Entry 4360

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4360

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Title:
1H, 13C and 15N Chemical Shift Assignments for the C2B-domain of Rabphilin 3
Deposition date:
1999-06-18
Original release date:
1999-09-10
Authors:
Ubach, Josep; Garcia, Jesus; Nittler, M. Paige; Sudhof, Thomas; Rizo, Josep
Citation:

Citation: Ubach, Josep; Garcia, Jesus; Nittler, M. Paige; Sudhof, Thomas; Rizo, Josep. "Structure of the Janus-faced C2B Domain of Rabphilin"  Nature Cell Biol. 1, 106-112 (1999).

Assembly members:

Assembly members:
rabphilin_3_C2B, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rabphilin_3_C2B: RGKILVSLMYSTQQGGLIVG IIRCVHLAAMDANGYSDPFV KLWLKPDMGKKAKHKTQIKK KTLNPEFNEEFFYDIKHSDL AKKSLDISVWDYDIGKSNDY IGGCQLGISAKGERLKHWYE CLKNKDKKIERWHQLQNENH

Data sets:
Data typeCount
13C chemical shifts561
15N chemical shifts153
1H chemical shifts1022

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rabphilin_3_C2B1

Entities:

Entity 1, rabphilin_3_C2B 140 residues - Formula weight is not available

1   ARGGLYLYSILELEUVALSERLEUMETTYR
2   SERTHRGLNGLNGLYGLYLEUILEVALGLY
3   ILEILEARGCYSVALHISLEUALAALAMET
4   ASPALAASNGLYTYRSERASPPROPHEVAL
5   LYSLEUTRPLEULYSPROASPMETGLYLYS
6   LYSALALYSHISLYSTHRGLNILELYSLYS
7   LYSTHRLEUASNPROGLUPHEASNGLUGLU
8   PHEPHETYRASPILELYSHISSERASPLEU
9   ALALYSLYSSERLEUASPILESERVALTRP
10   ASPTYRASPILEGLYLYSSERASNASPTYR
11   ILEGLYGLYCYSGLNLEUGLYILESERALA
12   LYSGLYGLUARGLEULYSHISTRPTYRGLU
13   CYSLEULYSASNLYSASPLYSLYSILEGLU
14   ARGTRPHISGLNLEUGLNASNGLUASNHIS

Samples:

sample_one: rabphilin_3_C2B, [U-99% 15N], 1.2 mM

sample_two: rabphilin_3_C2B, [U-99% 13C; U-99% 15N], 0.8 mM

sample_three: rabphilin_3_C2B, [U-99% 15N], 1.0 mM

sample_conditions_one: ionic strength: 0.17 .; pH: 6.1; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQF-COSYsample_onenot availablesample_conditions_one
2D 1H-1H NOESYsample_onenot availablesample_conditions_one
2D 1H-1H TOCSYsample_onenot availablesample_conditions_one
2D 1H-15N HSQCsample_onenot availablesample_conditions_one
HNCOsample_onenot availablesample_conditions_one
1H-15N NOESY-HSQCsample_onenot availablesample_conditions_one
1H-15N TOCSY-HSQCsample_onenot availablesample_conditions_one
HNCACBsample_onenot availablesample_conditions_one
(H)CBCACO(CA)HAsample_onenot availablesample_conditions_one
(H)C(CO)NH-TOCSYsample_onenot availablesample_conditions_one
H(C)(CO)NH-TOCSYsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
HNHAsample_onenot availablesample_conditions_one

Software:

NMRView v3.1 - peak-picking

NMRPipe - data processing

NMR spectrometers:

  • Varian 600 INOVA 600 MHz

Related Database Links:

PDB
GB EPQ01947
REF XP_003907251

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks