BMRB Entry 4351

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4351
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Title:
A 30-residue Fragment of the Carp Granulin-1 Protein Folds into a Stack of two Beta-hairpins Similar to that found in the Native Protein
Deposition date:
1999-06-03
Original release date:
2001-03-15
Authors:
Vranken, Wim; Chen, Z.; Xu, Ping; James, Susan; Bennett, Hugh; Ni, Feng
Citation:

Citation: Vranken, Wim; Chen, Z.; Xu, Ping; James, Susan; Bennett, Hugh; Ni, Feng. "A 30-residue Fragment of the Carp Granulin-1 Protein Folds into a Stack of two Beta-hairpins Similar to that found in the Native Protein"  J. Pept. Res. 53, 590-597 (1999).
PubMed: 10424355

Assembly members:

Assembly members:
granulin, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: carp   Taxonomy ID: 7962   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Cyprinus carpio

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
granulin: VIHCDAATICPDGTTCSLSP YGVWYCSPFS

Data sets:
Data typeCount
13C chemical shifts53
1H chemical shifts181
coupling constants5

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GRN1 CYPCA1

Entities:

Entity 1, GRN1 CYPCA 30 residues - Formula weight is not available

1   VALILEHISCYSASPALAALATHRILECYS
2   PROASPGLYTHRTHRCYSSERLEUSERPRO
3   TYRGLYVALTRPTYRCYSSERPROPHESER

Samples:

water_sample: granulin 0.54 mM

standard: ionic strength: 0.02 M; pH: 4.5; pressure: 1 atm; temperature: 288.0 K

Experiments:

NameSampleSample stateSample conditions
TOCSYwater_samplenot availablestandard
NOESYwater_samplenot availablestandard
TOCSY spinlock with MLEV-17water_samplenot availablestandard
NOESY using water flipbackwater_samplenot availablestandard
Water suppression with WATERGATEwater_samplenot availablestandard

Software:

XWIN-NMR - Spectrum processing

PRONTO - Assignment of spectra and keeping record of NOE contacts

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

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