BMRB Entry 4345
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4345
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Citation: Mao, Hongyuan; White, Susan; Williamson, james. "A Novel Loop-loop Recognition Motif in the Yeast Ribosomal Protein L30
Autoregulatory RNA Complex" Nat. Struct. Biol. 6, 1139-1147 (1999).
Assembly members:
YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30, polymer, 104 residues, 11284 Da.
L30N, polymer, 33 residues, 10814 Da.
Natural source: Common Name: BAKER'S YEAST Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: SACCHAROMYCES CEREVISIAE
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI Vector: PMALC-L30
Entity Sequences (FASTA):
YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30: APVKSQESINQKLALVIKSG
KYTLGYKSTVKSLRQGKSKL
IIIAANTPVLRKSELEYYAM
LSKTKVYYFQGGNNELGTAV
GKLFRVGVVSILEAGDSDIL
TTLA
L30N: GGACCGGAGUGUCGCAAGAC
GCAGAGAUGGUCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 648 |
| 15N chemical shifts | 138 |
| 1H chemical shifts | 1009 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | L30 PROTEIN | 1 |
| 2 | L30 MRNA | 2 |
Entities:
Entity 1, L30 PROTEIN 104 residues - 11284 Da.
| 1 | ALA | PRO | VAL | LYS | SER | GLN | GLU | SER | ILE | ASN | ||||
| 2 | GLN | LYS | LEU | ALA | LEU | VAL | ILE | LYS | SER | GLY | ||||
| 3 | LYS | TYR | THR | LEU | GLY | TYR | LYS | SER | THR | VAL | ||||
| 4 | LYS | SER | LEU | ARG | GLN | GLY | LYS | SER | LYS | LEU | ||||
| 5 | ILE | ILE | ILE | ALA | ALA | ASN | THR | PRO | VAL | LEU | ||||
| 6 | ARG | LYS | SER | GLU | LEU | GLU | TYR | TYR | ALA | MET | ||||
| 7 | LEU | SER | LYS | THR | LYS | VAL | TYR | TYR | PHE | GLN | ||||
| 8 | GLY | GLY | ASN | ASN | GLU | LEU | GLY | THR | ALA | VAL | ||||
| 9 | GLY | LYS | LEU | PHE | ARG | VAL | GLY | VAL | VAL | SER | ||||
| 10 | ILE | LEU | GLU | ALA | GLY | ASP | SER | ASP | ILE | LEU | ||||
| 11 | THR | THR | LEU | ALA |
Entity 2, L30 MRNA 33 residues - 10814 Da.
| 1 | G | G | A | C | C | G | G | A | G | U | ||||
| 2 | G | U | C | G | C | A | A | G | A | C | ||||
| 3 | G | C | A | G | A | G | A | U | G | G | ||||
| 4 | U | C | C |
Samples:
SAMPLE_1: YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30, [U-15N], 1.3 mM; L30N 1.3 mM
SAMPLE_2: YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30, [U-99% 13C; U-99% 15N], 1.7 mM; L30N 1.3 mM
SAMPLE_3: YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30, [U-99% 13C; U-99% 15N], 0.9 mM; L30N 1.3 mM
SAMPLE_4: YEAST (SACCHAROMYCES CEREVISIAE) RIBOSOMAL PROTEIN L30 0.5 mM; L30N, [U-99% 13C; U-99% 15N]-G, 1.3 mM
Ex-cond_1: ionic strength: 0.10 M; pH: 6.5; temperature: 303 K
Ex-cond_2: ionic_strength: 0.10 M; pH: 6.0 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
NMRPIPE -
NMR spectrometers:
- VARIAN INOVA 750 MHz
- VARIAN INOVA 600 MHz