BMRB Entry 4343

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4343

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Title:
Resonance assignment and topology of a 22 kDa C-terminal fragment of the polypyrimidine tract binding protein (PTB) containing two RNA binding domains
Deposition date:
1999-05-02
Original release date:
2003-01-06
Authors:
Conte, Maria Rosaria; Grune, Tim; Curry, Stephen; Matthews, Stephen
Citation:

Citation: Conte, Maria Rosaria; Grune, Tim; Curry, Stephen; Matthews, Stephen. "Letter to the Editor: Resonance assignment and topology of a 22 kDa C-terminal fragment of the polypyrimidine tract binding protein (PTB) containing two RNA binding domains"  J. Biomol. NMR 14, 383-384 (1999).

Assembly members:

Assembly members:
Polypyrimidien tract binding protein, polymer, 198 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET containing the eae gene

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Polypyrimidien tract binding protein: MGNSVLLVSNLNPERVTPQS LFILFGVYGDVQRVKILFNK KENALVQMADGNQAQLAMSH LNGHKLHGKPIRITLSKHQN VQLPREGQEDQGLTKDYGNS PLHRFKKPGSKNFQNIFPPS ATLHLSNIPPSVSEEDLKVL FSSNGGVVKGFKFFQKDRKM ALIQMGSVEEAVQALIDLHN HDLGENHHLRVSFSKSTI

Data sets:
Data typeCount
13C chemical shifts520
15N chemical shifts172
1H chemical shifts561

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA binding domain of PTB1

Entities:

Entity 1, RNA binding domain of PTB 198 residues - Formula weight is not available

1   METGLYASNSERVALLEULEUVALSERASN
2   LEUASNPROGLUARGVALTHRPROGLNSER
3   LEUPHEILELEUPHEGLYVALTYRGLYASP
4   VALGLNARGVALLYSILELEUPHEASNLYS
5   LYSGLUASNALALEUVALGLNMETALAASP
6   GLYASNGLNALAGLNLEUALAMETSERHIS
7   LEUASNGLYHISLYSLEUHISGLYLYSPRO
8   ILEARGILETHRLEUSERLYSHISGLNASN
9   VALGLNLEUPROARGGLUGLYGLNGLUASP
10   GLNGLYLEUTHRLYSASPTYRGLYASNSER
11   PROLEUHISARGPHELYSLYSPROGLYSER
12   LYSASNPHEGLNASNILEPHEPROPROSER
13   ALATHRLEUHISLEUSERASNILEPROPRO
14   SERVALSERGLUGLUASPLEULYSVALLEU
15   PHESERSERASNGLYGLYVALVALLYSGLY
16   PHELYSPHEPHEGLNLYSASPARGLYSMET
17   ALALEUILEGLNMETGLYSERVALGLUGLU
18   ALAVALGLNALALEUILEASPLEUHISASN
19   HISASPLEUGLYGLUASNHISHISLEUARG
20   VALSERPHESERLYSSERTHRILE

Samples:

sample: Polypyrimidien tract binding protein, [U-100% 15N; U-100% 13C], 1.0 mM; H2O 90%; D2O 10%

reporting_conditions: pH: 5.2; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAI46909
EMBL CAA43056 CAA43973 CAA46443 CAA46444 CAA47386
GB AAC99798 AAH02397 AAH04383 AAH13694 AAP35465
REF NP_002810 NP_114367 NP_114368 XP_001092088 XP_001092214
SP P26599
AlphaFold P26599

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks