BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4330

Title: A Single-Chain T Cell Receptor

Deposition date: 1999-04-09 Original release date: 2005-01-21

Authors: Hare, B.; Wyss, D.; Reinherz, E.; Wagner, G.

Citation: Hare, B.; Wyss, D.; Osburne, M.; Kern, P.; Reinherz, E.; Wagner, G.. "Structure, Specificity and CDR Mobility of a Class II Restricted T Cell Receptor."  Nat. Struct. Biol. 6, 574-581 (1999).

Assembly members:
ALPHA-BETA T CELL RECEPTOR (TCR) (D10), polymer, 249 residues, 27762 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology' 'Escherichia coli' 'E. coli' . . Escherichia coli 'BL21 (DE3)   Vector: 4330

Entity Sequences (FASTA):
ALPHA-BETA T CELL RECEPTOR (TCR) (D10): AVTQSPRNKVAVTGGKVTLS CNQTNNHNNMYWYRQDTGHG LRLIHYSYGAGSTEKGDIPD GYKASRPSQENFSLILELAT PSQTSVYFCASGGQGRAEQF FGPGTRLTVLGSDYKDDDDK RSGGGGSGGGGSGGSGAQQQ VRQSPQSLTVWEGETTILNC SYEDSTFDYFPWYRQFPGKS PALLIAISLVSNKKEDGRFT IFFNKREKKLSLHITDSQPG DSATYFCAATGSFNKLTFGA GTRLAVSPY

Data sets:
Data typeCount
13C chemical shifts107
15N chemical shifts209
1H chemical shifts987

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TCR ab1

Entities:

Entity 1, TCR ab 249 residues - 27762 Da.

1   ALAVALTHRGLNSERPROARGASNLYSVAL
2   ALAVALTHRGLYGLYLYSVALTHRLEUSER
3   CYSASNGLNTHRASNASNHISASNASNMET
4   TYRTRPTYRARGGLNASPTHRGLYHISGLY
5   LEUARGLEUILEHISTYRSERTYRGLYALA
6   GLYSERTHRGLULYSGLYASPILEPROASP
7   GLYTYRLYSALASERARGPROSERGLNGLU
8   ASNPHESERLEUILELEUGLULEUALATHR
9   PROSERGLNTHRSERVALTYRPHECYSALA
10   SERGLYGLYGLNGLYARGALAGLUGLNPHE
11   PHEGLYPROGLYTHRARGLEUTHRVALLEU
12   GLYSERASPTYRLYSASPASPASPASPLYS
13   ARGSERGLYGLYGLYGLYSERGLYGLYGLY
14   GLYSERGLYGLYSERGLYALAGLNGLNGLN
15   VALARGGLNSERPROGLNSERLEUTHRVAL
16   TRPGLUGLYGLUTHRTHRILELEUASNCYS
17   SERTYRGLUASPSERTHRPHEASPTYRPHE
18   PROTRPTYRARGGLNPHEPROGLYLYSSER
19   PROALALEULEUILEALAILESERLEUVAL
20   SERASNLYSLYSGLUASPGLYARGPHETHR
21   ILEPHEPHEASNLYSARGGLULYSLYSLEU
22   SERLEUHISILETHRASPSERGLNPROGLY
23   ASPSERALATHRTYRPHECYSALAALATHR
24   GLYSERPHEASNLYSLEUTHRPHEGLYALA
25   GLYTHRARGLEUALAVALSERPROTYR

Samples:

sample_one: ALPHA-BETA T CELL RECEPTOR (TCR) (D10) 1.0 mM; sodium acetate 20 mM

sample_two: ALPHA-BETA T CELL RECEPTOR (TCR) (D10)' '[U-50% 2H]; .

sample_conditions: ionic strength: 20 mM; pH: 5.0 na; pressure: 1.0 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 750 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts