BMRB Entry 4329

Title:
Sequential Assignment and Secondary Structure Analysis of the NADP(H)-Binding Domain of Escherichia coli Transhydrogenase
Deposition date:
1999-04-09
Original release date:
1999-10-26
Authors:
Johansson, Carina; Bergkvist, Anders; Fjellstrom, Ola; Rydstrom, Jan; Karlsson, Goran
Citation:

Citation: Johansson, Carina; Bergkvist, Anders; Fjellstrom, Ola; Rydstrom, Jan; Karlsson, Goran. "Sequential Assignment and Secondary Structure Analysis of the NADP(H)-Binding Domain of Escherichia coli Transhydrogenase"  J. Biomol. NMR 14, 295-296 (1999).

Assembly members:

Assembly members:
E.coli transhydrogenase domain III, polymer, 186 residues, 20393 Da.
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, non-polymer, 743.405 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts164
1H chemical shifts344

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ecIII1
2NADP+2

Entities:

Entity 1, ecIII 186 residues - 20393 Da.

1   METHISHISHISHISHISHISSERSERGLN
2   GLUVALGLYGLUHISARGGLUILETHRALA
3   GLUGLUTHRALAGLULEULEULYSASNSER
4   HISSERVALILEILETHRPROGLYTYRGLY
5   METALAVALALAGLNALAGLNTYRPROVAL
6   ALAGLUILETHRGLULYSLEUARGALAARG
7   GLYILEASNVALARGPHEGLYILEHISPRO
8   VALALAGLYARGLEUPROGLYHISMETASN
9   VALLEULEUALAGLUALALYSVALPROTYR
10   ASPILEVALLEUGLUMETASPGLUILEASN
11   ASPASPPHEALAASPTHRASPTHRVALLEU
12   VALILEGLYALAASNASPTHRVALASNPRO
13   ALAALAGLNASPASPPROLYSSERPROILE
14   ALAGLYMETPROVALLEUGLUVALTRPLYS
15   ALAGLNASNVALILEVALPHELYSARGSER
16   METASNTHRGLYTYRALAGLYVALGLNASN
17   PROLEUPHEPHELYSGLUASNTHRHISMET
18   LEUPHEGLYASPALALYSALASERVALASP
19   ALAILELEULYSALALEU

Entity 2, NADP+ - C21 H28 N7 O17 P3 - 743.405 Da.

1   NAP

Samples:

sample_one: E.coli transhydrogenase domain III0.9 – 1.5 mM

sample_conditions: pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_onenot availablesample_conditions
1H-15N TOCSYsample_onenot availablesample_conditions
HNCAsample_onenot availablesample_conditions
HN(CO)CAsample_onenot availablesample_conditions
HNCOsample_onenot availablesample_conditions
HCACOsample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity INOVA 800 MHz
  • Varian Unity INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA15336 BAB35731 BAG77247 BAI25577 BAI30551
EMBL CAA46885 CAB37090 CAD01834 CAP76101 CAQ32078
GB AAC74674 AAG56589 AAL20399 AAN43206 AAN80454
PIR AI0682
REF NP_310335 NP_416119 NP_456000 NP_460440 NP_707499
SP P0AB67 P0AB68 P0AB69 P0AB70
AlphaFold P0AB67 P0AB68 P0AB69 P0AB70

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks