BMRB Entry 4326

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4326

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal Domain of DNA Polymerase B (residues 2-87).

Deposition date:

1999-03-25

Original release date:

1999-05-19

Authors:

Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory

Citation:

Citation: Liu, Dingjiang; DeRose, Eugene; Prasad, Rajendra; Wilson, Samuel; Mullen, Gregory. "Assignments of 1H, 15N, and 13C Resonances for the Backbone and Side Chains of the N-Terminal Domain of DNA Polymerase B. Determination of the Secondary Structure and Tertiary Contacts" Biochemistry 33, 9537-9545 (1994).

Assembly members:

Assembly members:
DNA Polymerase B, polymer, 87 residues, 9490 Da.

Natural source:

Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET-8k

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA Polymerase B: MSKRKAPQETLNGGITDMLV ELANFEKNVSQAIHKYNAYR KAASVIAKYPHKIKSGAEAK KLPGVGTKIAEKIDEFLATG KLRKLEK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	312
15N chemical shifts	83
1H chemical shifts	598

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	B-Pol N-terminal Domain	1

Entities:

Entity 1, B-Pol N-terminal Domain 87 residues - 9490 Da.

1

MET

SER

LYS

ARG

LYS

ALA

PRO

GLN

GLU

THR

2

LEU

ASN

GLY

ILE

THR

ASP

MET

LEU

VAL

3

GLU

LEU

ALA

ASN

PHE

GLU

LYS

ASN

VAL

SER

4

GLN

ALA

ILE

HIS

LYS

TYR

ASN

ALA

TYR

ARG

5

LYS

ALA

SER

VAL

ILE

ALA

LYS

TYR

PRO

6

HIS

LYS

ILE

LYS

SER

GLY

ALA

GLU

ALA

LYS

7

LYS

LEU

PRO

GLY

VAL

GLY

THR

LYS

ILE

ALA

8

GLU

LYS

ILE

ASP

GLU

PHE

LEU

ALA

THR

GLY

9

LYS

LEU

ARG

LYS

LEU

GLU

LYS

Samples:

sample_one: DNA Polymerase B, [U-100% 13C; U-100% 15N], 2.8 mM; Tris-d11 5 mM; NaCl 400 mM

sample_two: DNA Polymerase B, [U-100% 15N], 4.7 mM; Tris-d11 5 mM; NaCl 400 mM

sample_three: DNA Polymerase B 1.4 mM; Tris-d11 5 mM; NaCl 400 mM

sample_conditions: ionic strength: 0.800 .; pH: 6.7 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	not available	not available	sample_conditions
2D 1H-1H TOCSY	not available	not available	sample_conditions
2D 1H-1H DQF-COSY	not available	not available	sample_conditions
2D 1H-15N HMQC	not available	not available	sample_conditions
2D 1H-15N HMQC-J	not available	not available	sample_conditions
3D 1H-15N-1H NOESY-HMQC	not available	not available	sample_conditions
3D 1H-15N-1H TOCSY-HMQC	not available	not available	sample_conditions
3D 1H-13C-1H TOCSY-HMQC	not available	not available	sample_conditions
3D HNCA	not available	not available	sample_conditions
3D HN(CO)CA	not available	not available	sample_conditions
3D HNCO	not available	not available	sample_conditions
3D 1H-13C-1H NOESY-HMQC	not available	not available	sample_conditions

Software:

Felix v2.10 - processing and analysis of spectra

NMR spectrometers:

General Electric GN-500 500 MHz

Related Database Links:

BMRB	18267
PDB	1BNO 1BNP 1BPD 1BPE 1BPX 1BPY 1BPZ 1DK2 1DK3 1HUO 1HUZ 1MQ2 1MQ3 1TV9 1TVA 1ZJM 1ZJN 1ZQA 1ZQB 1ZQC 1ZQD 1ZQE 1ZQF 1ZQG 1ZQH 1ZQI 1ZQJ 1ZQK 1ZQL 1ZQM 1ZQN 1ZQO 1ZQP 1ZQQ 1ZQR 1ZQS 1ZQT 2BPF 2BPG 2FMP 2FMQ 2FMS 2I9G 2ISO 2ISP 2P66 2PXI 3C2K 3C2L 3C2M 3GDX 3ISB 3ISC 3ISD 3JPN 3JPO 3JPP 3JPQ 3JPR 3JPS 3JPT 3LK9 3MBY 3OGU 3RH4 3RH5 3RH6 3RJE 3RJF 3RJG 3RJH 3RJI 3RJJ 3RJK 3TFR 3TFS 3UXN 3UXO 3UXP 3V72 3V7J 3V7K 3V7L 4DO9 4DOA 4DOB 4DOC 4F5N 4F5O 4F5P 4F5Q 4F5R 4GXI 4GXJ 4GXK 4JWM 4JWN 4KLD 4KLE 4KLF 4KLG 4KLH 4KLI 4KLJ 4KLL 4KLM 4KLO 4KLQ 4KLS 4KLT 4KLU 4LVS 4M2Y 4M47 4M9G 4M9H 4M9J 4M9L 4M9N 4MF2 4MF8 4MFA 4MFC 4MFF 4NLK 4NLN 4NLZ 4NM1 4NM2 4NXZ 4NY8 4O5C 4O5E 4O5K 4O9M 4P2H 4PGQ 4PGX 4PGY 4PH5 4PHA 4PHD 4PHE 4PHP 4PPX 4R63 4R64 4R65 4R66 4RPX 4RPY 4RPZ 4RQ0 4RQ1 4RQ2 4RQ3 4RQ4 4RQ5 4RQ6 4RQ7 4RQ8 4RT2 4RT3 4TUP 4TUQ 4TUR 4TUS 4UAW 4UAY 4UAZ 4UB1 4UB2 4UB3 4UB4 4UB5 4UBB 4UBC 5BOL 5BOM 5BPC 5DB6 5DB7 5DB8 5DB9 5DBA 5DBB 5DBC 7ICE 7ICF 7ICG 7ICH 7ICI 7ICJ 7ICK 7ICL 7ICM 7ICN 7ICO 7ICP 7ICQ 7ICR 7ICS 7ICT 7ICU 7ICV 8ICA 8ICB 8ICC 8ICE 8ICF 8ICG 8ICH 8ICI 8ICJ 8ICK 8ICL 8ICM 8ICN 8ICO 8ICP 8ICQ 8ICR 8ICS 8ICT 8ICU 8ICV 8ICW 8ICX 8ICY 8ICZ 9ICA 9ICB 9ICC 9ICE 9ICF 9ICG 9ICH 9ICI 9ICJ 9ICK 9ICL 9ICM 9ICN 9ICO 9ICP 9ICQ 9ICR 9ICS 9ICT 9ICU 9ICV 9ICW 9ICX 9ICY
DBJ	BAA06099 BAC36630 BAD97257 BAE27405 BAE30399
EMBL	CAD29079 CAD29080 CAD29081 CAD29082 CAD29083
GB	AAA41900 AAA41901 AAA60133 AAB00389 AAB59441
REF	NP_001029936 NP_001244632 NP_001297194 NP_002681 NP_035260
SP	P06746 P06766 Q27958 Q8K409
TPG	DAA14453
AlphaFold	P06746 P06766 Q27958 Q8K409

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks