BMRB Entry 4312

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PDB ID: 1jm4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4312
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF

Deposition date:

1999-02-28

Original release date:

1999-03-03

Authors:

Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming

Citation:

Citation: Dhalluin, Christophe; Carlson, Justin; Zeng, Lei; He, Cheng; Aggarwal, Aneel; Zhou, Ming-Ming. "1H, 15N and 13C Resonance Assignments for the Bromodomain of the Histone Acetyltransferase hsP/CAF" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
histone acetyltransferase bromodomain, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
histone acetyltransferase bromodomain: GSHMSKEPRDPDQLYSTLKS ILQQVKSHQSAWPFMEPVKR TEAPGYYEVIRSPMDLKTMS ERLKNRYYVSKKLFMADLQR VFTNCKEYNAPESEYYKCAN ILEKFFFSKIKEAGLIDK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	382
15N chemical shifts	107
1H chemical shifts	708

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	brd subunit 1	1

Entities:

Entity 1, brd subunit 1 118 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

LYS

GLU

PRO

ARG

ASP

2

PRO

ASP

GLN

LEU

TYR

SER

THR

LEU

LYS

SER

3

ILE

LEU

GLN

VAL

LYS

SER

HIS

GLN

SER

4

ALA

TRP

PRO

PHE

MET

GLU

PRO

VAL

LYS

ARG

5

THR

GLU

ALA

PRO

GLY

TYR

GLU

VAL

ILE

6

ARG

SER

PRO

MET

ASP

LEU

LYS

THR

MET

SER

7

GLU

ARG

LEU

LYS

ASN

ARG

TYR

VAL

SER

8

LYS

LEU

PHE

MET

ALA

ASP

LEU

GLN

ARG

9

VAL

PHE

THR

ASN

CYS

LYS

GLU

TYR

ASN

ALA

10

PRO

GLU

SER

GLU

TYR

LYS

CYS

ALA

ASN

11

ILE

LEU

GLU

LYS

PHE

SER

LYS

ILE

12

LYS

GLU

ALA

GLY

LEU

ILE

ASP

LYS

Samples:

sample_1: histone acetyltransferase bromodomain, [U-13C; U-15N; U-75% 2H], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_2: histone acetyltransferase bromodomain, [U-13C; U-15N], 1.0 mM; phosphate buffer 100 mM; perdeuterated DTT 5 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

Ex-cond_1: pH: 6.5 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	Ex-cond_1
2D 1H-13C HSQC	not available	not available	Ex-cond_1
3D HNCACB	not available	not available	Ex-cond_1
3D HN(CO)CACB	not available	not available	Ex-cond_1
3D (H)C(CO)NH-TOCSY	not available	not available	Ex-cond_1
3D HNHA	not available	not available	Ex-cond_1
3D HCCH TOCSY	not available	not available	Ex-cond_1
15N edited TOCSY	not available	not available	Ex-cond_1
15N edited NOESY	not available	not available	Ex-cond_1
13C edited NOESY	not available	not available	Ex-cond_1

Software:

NMRPipe v3.1 - spectra Processing

NMRVIEW v3.1 - spectra analysis

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz